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M7257

Sigma-Aldrich

α-Mannosidase from Canavalia ensiformis (Jack bean)

ammonium sulfate suspension, ≥15 units/mg protein (biuret)

Synonym(s):

α-D-Mannoside mannohydrolase

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About This Item

CAS Number:
9025-42-7
Enzyme Commission number:
3.2.1.24 (BRENDA, IUBMB)
EC Number:
232-794-1
MDL number:
MFCD00130495
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

Canavalia ensiformis

Quality Level

200

form

ammonium sulfate suspension

specific activity

≥15 units/mg protein (biuret)

foreign activity

β-galactosidase, β-N-acetylglucosaminidase, α-galactosidase and α-L-fucosidase <0.05%

storage temp.

2-8°C

InChI

1S/Zn/q+2

InChI key

PTFCDOFLOPIGGS-UHFFFAOYSA-N

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General description

α−Mannosidase from Canavalia ensiformis is a tretamer composed of two subunits that each contain two components at 44 and 66 kDa.
α-Mannosidase is an exo-glycosidase and an inc metalloenzyme.
Lysosomal α-mannosidases are class II mannosidases that belong to glycoside hydrolase family 38 and play an important role in the degradation of asparagine-linked carbohydrates of glycoproteins.

Application

α-Mannosidase can be used to liberate mannose from a variety of synthetic and natural α-mannosides. It has also been used in a study to investigate the causes of neurodegeneration in mucolipidosis II ′knock-in′ mice.
α-Mannosidase from Canavalia ensiformis (Jack bean) has been used:
  • for the digestion of neutral glycans.
  • to remove O-glycans from recombinant β-glucosidase
  • for the inhibition of renal fibroblasts proliferation

Liberates mannose from a variety of synthetic and natural α-mannosides.

Biochem/physiol Actions

α−Mannosidase has been shown to inhibit the proliferation of B-lymphocytes.
α-Mannosidase cleaves near α1−2-, α1−3- and α1−6-linked mannose residues.
α-Mannosidase is an acid hydrolase which is located in plant vacuoles and is thought to be involved with the turnover of N-linked glycoproteins.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl α-D-mannoside to p-nitrophenol and D-mannose per min at pH 4.5 at 25°C.

Physical form

Suspension in 3.0 M (NH4)2SO4 and 0.1 mM zinc acetate, pH 7.5

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Engineering a highly active thermophilic beta-glucosidase to enhance its pH stability and saccharification performance
Xia W, et al.
Biotechnology for Biofuels, 9(1), 147-147 (2016)
Jack bean alpha-mannosidase: Amino acid sequencing and N-glycosylation analysis of a valuable glycomics tool.
Kumar G, et al.
Glycobiology, 24(3) (2014)
W Einhoff et al.
Biological chemistry Hoppe-Seyler, 369(3), 165-169 (1988-03-01)
The alpha-mannosidase from Canavalia ensiformis was characterized with respect to molecular mass, glycoprotein nature, amino-acid composition, enzymic properties and action on animal cells. The enzyme is composed of two pairs of subunits (molecular mass 44 and 66 kDa) which form
Y Kimura et al.
European journal of biochemistry, 264(1), 168-175 (1999-08-14)
The acid hydrolase alpha-mannosidase, which accumulates in plant vacuoles and probably is involved in the catabolism and turnover of N-linked glycoproteins, is itself a glycoprotein with at least one high-mannose-type and one complex-type N-glycan. The puzzling finding that alpha-mannosidase stably
Reto Buser et al.
The Journal of biological chemistry, 285(14), 10715-10723 (2010-01-12)
Coprinopsis cinerea is a model organism for fruiting body development in homobasidiomycetes. Here, we focused on N-linked oligosaccharides (NLO) of cell wall proteins in the hyphae of two developmental stages, vegetative mycelium and fruiting body. High mannose-type glycans were the
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