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NXTRACT

Sigma-Aldrich

NuCLEAR™ Extraction Kit

For mammalian tissue or cultured cells

Sinónimos:

Nuclear Isolation Kit

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About This Item

Código UNSPSC:
41105517
NACRES:
NA.56

Nivel de calidad

300

uso

 kit sufficient for 10 extractions (1 ml packed cell volume)
 kit sufficient for 100 extractions (100 μl packed cell volume)

técnicas

protein extraction: suitable
western blot: suitable

Condiciones de envío

dry ice

temp. de almacenamiento

−20°C

Categorías relacionadas

Descripción general

The procedure for the nuclear protein extraction method is to allow cells to swell with hypotonic buffer. The cells are then disrupted, the cytoplasmic fraction is removed, and the nuclear proteins are released from the nuclei by a high salt buffer.

Aplicación

NXTRACT kit was used to study the impact of salt on cardiac differential gene expression and coronary lesion in normotensive mineralocorticoid-treated mice. It was also used to test the therapeutic potential of andrographolide for treating endometriosis.

Otras notas

Within this kit is a complete system for preparing nuclear and cytoplasmic protein extracts from mammalian tissue or cultured cells. All reagents necessary for extraction are included.
A number of different procedures in the detailed technical bulletin enable the selection that best fits a particular application. For example, choose between detergent and non-detergent extraction of nuclear protein or between the standard hypotonic lysis buffer for most cell types and isotonic lysis buffer for fragile cells. In addition, the kit provides a procedure for salt reduction from the nuclear extract with dilution buffer. NuCLEAR offers the flexiblity you need for optimal protein extraction. Extracts can be prepared in less than 2 hours and are highly pure since there is little or no cross-contamination between nuclear and cytoplasmic extracts.

Ligadura / enlace

Recommended Antibodies for Immunodetection L1293, N2662, AMAB90549

Información legal

NuCLEAR is a trademark of Sigma-Aldrich Co. LLC

Los componentes del kit también están disponibles por separado

Referencia del producto
Descripción
SDS
  • 3× Dilution and Equilibration Buffer 90 mL
  • P8340Protease Inhibitor Cocktail 1 mLSDS

Pictogramas

Corrosion
GHS05

Palabra de señalización

Danger

Frases de peligro

H290,H314

Clasificaciones de peligro

Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A

Código de clase de almacenamiento

8A - Combustible corrosive hazardous materials

Punto de inflamabilidad (°F)

188.6 °F - closed cup

Punto de inflamabilidad (°C)

87 °C - closed cup

Elija entre una de las versiones más recientes:

Certificados de análisis (COA)

Lot/Batch Number
0000149724
0000149724
0000114896
059M4108V
11181117

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Fangyuan Dong et al.
EBioMedicine, 39, 472-483 (2018-12-12)
Accumulating evidence has revealed the pivotal role of epigenetic regulation in the pathogenesis of liver disease. However, the epigenetic mechanism that accounts for hepatic stellate cells (HSCs) activation in liver fibrosis remains largely unknown. Primary HSCs were used to screen
Isolation of intact nuclei for nuclear extract preparation from a fragile B-lymphocyte cell line.
R B Dyer et al.
BioTechniques, 19(2), 192-195 (1995-08-01)
Qing Wang et al.
American journal of physiology. Regulatory, integrative and comparative physiology, 302(9), R1025-R1033 (2012-03-10)
We previously reported that excess of deoxycorticosterone-acetate (DOCA)/salt-induced cardiac hypertrophy in the absence of hypertension in one-renin gene mice. This model allows us to study molecular mechanisms of high-salt intake in the development of cardiovascular remodeling, independently of blood pressure
K A Lee et al.
Gene analysis techniques, 5(2), 22-31 (1988-03-01)
A convenient and rapid method for preparing soluble extracts from the nuclei of as few as 3 x 10(7) mammalian cells (miniextract procedure) is described. By several criteria, miniextracts are comparable to nuclear extracts prepared from large numbers of cells
Sumie Hiramatsu et al.
Scientific reports, 9(1), 3054-3054 (2019-03-01)
Global DNA hypomethylation in CD4+ cells in systemic lupus erythematosus (SLE) was suggested to play a key role in the pathogenesis. To identify new methylation-sensitive genes, we integrated genome-wide DNA methylation and mRNA profiling data in CD4+ cells of MRL/lpr
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