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CELLYTPN1

Sigma-Aldrich

CelLytic™ PN Isolation/Extraction Kit

For plant leaves

Sinónimos:

Cell lysis kit

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About This Item

Código UNSPSC:
41116134
NACRES:
NA.56

Nivel de calidad

200

uso

 kit sufficient for 30 extractions (20 g of fresh or frozen leaves)

técnicas

ChIP: suitable

Condiciones de envío

wet ice

temp. de almacenamiento

2-8°C

Descripción general

CelLytic™-PN extraction kit is for the isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from few grams to hundreds grams of fresh and frozen leaves. The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA and histones.

Aplicación

The nuclear protein extract is suitable for the detection of DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis as well as Western blot assay and similar techniques. The isolated nuclei can also be used as a source for chromatin, genomic DNA, RNA, etc. The kit provides a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis.

Características y beneficios

Efficient Nuclei Isolation and Protein Extraction: CelLytic™ PN Isolation/Extraction Kit provides a fast and efficient method for isolating nuclei and extracting functional nuclear proteins from plant leaves, saving time and effort for academic researchers.

Versatility: The isolated nuclei can be used as a source for chromatin, genomic DNA, RNA, etc., making CelLytic™ PN Isolation/Extraction Kit a versatile solution for plant molecular biology research.

Suitable for Detection of DNA-Protein Interactions: The nuclear protein extract obtained using this kit is ideal for detecting DNA-protein interactions using gel-shift assay, DNase-I footprinting analysis, western blot assay, and similar techniques, which is crucial for researchers studying transcription factors and chromatin remodeling.

Plant Model Specific Protocol: The CelLytic™ PN Isolation/Extraction Kit offers a detailed protocol for nuclei isolation and protein extraction from four plant models: tobacco, tomato, spinach, and Arabidopsis, making it an easy-to-follow solution for researchers working with these plant models.

Rapid Extraction: The CelLytic™ PN Isolation/Extraction Kit provides academic researchers with a rapid extraction method, allowing them to quickly extract functional nuclear proteins from plant leaves for their research.

Otras notas

This kit is for the rapid isolation of nuclei and extraction of functional nuclear proteins from plant leaves. Nuclei or nuclear proteins can be extracted from a few grams to hundreds of grams of fresh and frozen leaves.

Información legal

CelLytic is a trademark of Sigma-Aldrich Co. LLC

Producto relacionado

Referencia del producto
Descripción
Precios

08168

Timestrip Plus™ 8 °C

Palabra de señalización

Danger

Frases de peligro

H302,H315,H318,H410

Clasificaciones de peligro

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Skin Irrit. 2

Código de clase de almacenamiento

10 - Combustible liquids

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Certificados de análisis (COA)

Lot/Batch Number
0000405906
0000405906
0000299132
0000299132
0000266316

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Xu Zhang et al.
Journal of experimental botany, 66(9), 2709-2721 (2015-03-20)
The mycotoxin fumonisin B1 (FB1) is a strong inducer of programmed cell death (PCD) in plants, but its underlying mechanism remains unclear. Here, we describe two ubiquitin ligases, RING DOMAIN LIGASE3 (RGLG3) and RGLG4, which control FB1-triggered PCD by modulating
Guoyin Liu et al.
Plant physiology and biochemistry : PPB, 156, 155-166 (2020-09-20)
WRKY transcription factors play key roles in plant biotic and abiotic stress responses, but the function of some MaWRKYs remains elusive. Here, we characterized the positive role of MaWRKY80 in drought stress resistance and the underlying mechanism. MaWRKY80 was significantly
Lei Wang et al.
The EMBO journal, 29(11), 1903-1915 (2010-04-22)
Many core oscillator components of the circadian clock are nuclear localized but how the phase and rate of their entry contribute to clock function is unknown. TOC1/PRR1, a pseudoresponse regulator (PRR) protein, is a central element in one of the
Xiaosa Xu et al.
Developmental cell, 56(4), 557-568 (2021-01-06)
Crop productivity depends on activity of meristems that produce optimized plant architectures, including that of the maize ear. A comprehensive understanding of development requires insight into the full diversity of cell types and developmental domains and the gene networks required
Irfan Ullah Khan et al.
Frontiers in plant science, 11, 623-623 (2020-06-13)
Drought stress adversely affects plant growth and development and significantly reduces crop productivity and yields. The phytohormone abscisic acid (ABA) rapidly accumulates in response to drought stress and mediates the expression of stress-responsive genes that help the plant to survive
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