β-Lactamase (β-lactamase, βL, EC 3.5.2.6) is an enzyme first identified in Escherichia coli and has been described as penicillinase. A number of βLs have since been identified from various bacteria. β-Lactamases specifically hydrolyze β-lactam rings present in antibiotics such as penicillin, cephalosporins, monobactam, and carbapenem, and confer resistance against these antibiotics.
Application
Beta-Lactamase Inhibitor Screening Kit has been used in enzymatic assays.[1]
Features and Benefits
Compatible with high-throughput handling systems.
Suitability
Suitable for the screening of inhibitors of β-Lactamase
Principle
The β-Lactamase Inhibitor Screening Kit is a rapid, simple and sensitive assay that is suitable for high throughput screening of β-Lactamase inhibitors. βL activity is measured by hydrolyzing a chromogenic cephalosporin called nitrocefin, producing a colorimetric product (A490), proportional to the enzymatic activity present.
Applied and environmental microbiology, 85(3) (2018-11-28)
Metallo-β-lactamase (MBL)-producing Enterobacteriaceae are of grave clinical concern, particularly as there are no metallo-β-lactamase inhibitors approved for clinical use. The discovery and development of MBL inhibitors to restore the efficacy of available β-lactams are thus imperative. We investigated a zinc-chelating
Hello,
I want to use your β-lactamase inhibitor screening kit, however I could not find which β-lactamase(s) the kit includes, and where I can a step by step protocol
Included are nitrocefin, β-lactamase, a β-lactamase assay buffer, and the inhibitor control clavulanic acid. These are not sold as distinct products on the website.
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