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L7920

Sigma-Aldrich

β-Lactamase Blend

recombinant, expressed in E. coli

Synonym(s):

Carbapenemase, Cephalosprinase

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1 VIAL
€438.00

€438.00

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1 VIAL
€438.00

About This Item

Enzyme Commission number:
3.5.2.6 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54

€438.00

Please contact Customer Service for Availability

recombinant

expressed in E. coli

Quality Level

200

sterility

γ-irradiated

form

lyophilized powder

storage temp.

2-8°C

General description

No detectable growth in tryptone soy broth at 30-35 °C for 14 days

Biochem/physiol Actions

β-Lactamases exhibit high carbapenemase activity. β-lactamases can destroy the β-lactam ring.[1] True carbapenemases has the ability to hydrolyse several β-lactam ring containing carbapenems like meropenem, doripenem, imipenem and ertapenem.[2]

Components

600-1500 IU beta-lactamase I per vial
60-150 IU beta-lactamase II per vial

Unit Definition

One unit will hydrolyze 1.0 μmole substrate per min (βI: benzylpenicillin; βII: cephalosporin C) in phosphate buffer, pH 7.0 at 25°C.

Physical form

Lyophilized powder containing sodium chloride, potassium phosphate, dipotassium phosphate and sorbitol.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H317,H334

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number
SLCM4634
SLCQ2635
SLCM5001
SLCP4519
SLCM1748

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Mechanisms and Detection of Antimicrobial Resistance
Principles and Practice of Pediatric Infectious Diseases (Fourth Edition), 1467-1478 (2017)
Lisa M Miller et al.
ACS applied materials & interfaces, 11(36), 32599-32604 (2019-08-27)
Antimicrobial resistance (AMR) has been identified as a major threat to public health worldwide. To ensure appropriate use of existing antibiotics, rapid and reliable tests of AMR are necessary. One of the most common and clinically important forms of bacterial
Identification and screening of carbapenemase-producing Enterobacteriaceae
Nordmann P, et al.
Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases, 18(5), 432-438 (2012)
Matthew D Simmons et al.
Antibiotics (Basel, Switzerland), 9(10) (2020-10-03)
The misuse of antibiotics in health care has led to increasing levels of drug resistant infections (DRI's) occurring in the general population. Most technologies developed for the detection of DRI's typically focus on phenotyping or genotyping bacterial resistance rather than
Paolo Gaibani et al.
The new microbiologica, 37(1), 87-90 (2014-02-18)
We describe an outbreak of a carbapenemase-producing Klebsiella pneumoniae sequence type 258 (ST258) clone in an Italian hospital during two months in 2010. The rapid detection and management of the eleven patients colonized and infected with KPC-producing K.pneumoniae curbed the
View All Related Papers

Questions

1–2 of 2 Questions  

  1. Dear Mr/Ms, I ordered β-Lactamase Blend L7920 and I am interested in knowing the concentration in micrograms per mL. (Micrograms per mL=IU/mL/min×Conversion Factor). Can you please tell me what is the conversion factor? Regards, Glorjen

    1 answer

    1. This product is provided in lyophilized solid form with the unit activity reported in lot specific Certificate of Analysis.

      Using a representative lot, SLCQ2635 as an example, one vial contains 1289 units. The activity can be converted to mass in milligrams using the batch specific activity value of 124 units/mg.

      Thus, a vial from batch (SLCQ2635) contains 10.39 mg of enzyme; 1289 units x (1 mg / 124 unit) = 10.39 mg.

      The volume in mL would be dependent on the volume of solvent added. For example, adding 1 mL of solvent to a vial from this batch, would result in a concentration of 10.39 mg/mL.

      Helpful?

  2. HOW TO DISSOLVE THE VIAL

    1 answer

    1. This product has been sterilized by gamma-irradiaton and can be reconstituted with in 10 mL of deionized purified water, then filter sterilzed through a 0.2 micron low protein binding membrane. If being used for the preparation of agar plates, it should be added to the medium at a temperature of less than 55 deg C. After reconstitution, the solution is stable for 2 months at 2-8 deg C."

      Helpful?

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