GR11L
Anti-IGF-IR (Ab-1) Mouse mAb (αIR3)
lyophilized, clone αIR3, Calbiochem®
Synonym(s):
Anti-IGF-IR, Anti-Insulin-Like Growth Factor Receptor, Anti-Insulin-Like Growth Factor Receptor, Anti-IGF-IR
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
αIR3, monoclonal
form
lyophilized
does not contain
preservative
species reactivity
human
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
isotype
IgG1
shipped in
ambient
storage temp.
2-8°C
target post-translational modification
unmodified
Gene Information
human ... IGF1R(3480)
General description
This Anti-IGF-IR (Ab-1) Mouse mAb (αIR3) is validated for use in IF, IP, Neutralization Studies, Paraffin Sections, Immunoblotting for the detection of IGF-IR (Ab-1).
Application
Immunofluorescence (1-5 µg/ml)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (1 µg/ml; see application references)
Paraffin Sections (not recommended)
Immunoblotting (not recommended)
Immunoprecipitation (1 µg/sample)
Neutralization Studies (1 µg/ml; see application references)
Paraffin Sections (not recommended)
Immunoblotting (not recommended)
Warning
Toxicity: Standard Handling (A)
Physical form
Lyophilized from 20 mM ammonium bicarbonate solution, 100 µg BSA .
Reconstitution
Reconstitute with sterile phosphate buffered saline (PBS), pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to a final concentration of 100 µg/ml. Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least two h. Following reconstitution, aliquot and freeze (-20°C) or refrigerate (4°C) with 0.1% sodium azide. Freezing of aliquots is recommended for long term storage of reconstituted product. Avoid freeze/thaw cycles of solutions.
Other Notes
Blocks IGF-I binding to the IGF-I receptor. May also weakly cross-react with the insulin receptor (see application references). It also inhibits the growth of MCF-7 cells in culture suggesting the IGF-I receptor may be involved in autocrine regulation of cell growth (see application references). Antibody should be titrated for optimal results in individual systems.
Roth, R. 1988. Science239, 1269.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M. 1987. Science257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res.24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem.258, 6561.
Rohlik, Q.T., et al. 1987. Biochem. Biophy Res. Comm.149, 276.
Rosen, O.M. 1987. Science257, 1452.
Rechler, M.M., and Nissley, S.P. 1986. Hormone Res.24, 152.
Ullrich, A., et al. 1986. EMBO J.5, 2503.
Zapf, S., and Froesch, E.R. 1986. Hormone Res.24, 121.
Humbel, R.E. 1984. I. Chemistry in Li Hormonal proteins and peptides. Vol 12, Chap. 4 (Academic Press, New York, 1984).
Kull, F.C., et al.1983. J. Biol. Chem.258, 6561.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Daniela Kiepe et al.
Endocrinology, 149(10), 4901-4911 (2008-06-17)
The IGF/IGF binding protein (IGFBP) system is an important component in the hormonal regulation of longitudinal growth. Evidence from in vitro studies indicates that IGFBPs may have IGF-independent effects. We analyzed the biological activity of intact IGFBP-2 and defined carboxy-terminal
Nicole Bäumer et al.
Nature protocols, 11(1), 22-36 (2015-12-04)
Knockdown of genes by RNA interference (RNAi) in vitro requires methods of transfection or transduction, both of which have limited impact in vivo. As a virus-free approach, we chemically coupled cell surface receptors internalizing antibodies to the short interfering RNA
Roxana Schillaci et al.
British journal of haematology, 130(1), 58-66 (2005-06-29)
Chronic lymphocytic leukaemia (CLL) is characterized by the accumulation of long-lived B lymphocytes blocked in G(0/1) by impaired apoptosis. As insulin-like growth factor-I (IGF-I) is known for its antiapoptotic effects in different cell types, we investigated whether IGF-I and its
Tamara Aleksic et al.
Cancer research, 70(16), 6412-6419 (2010-08-17)
The type 1 insulin-like growth factor receptor (IGF-1R) is a transmembrane glycoprotein composed of two extracellular alpha subunits and two beta subunits with tyrosine kinase activity. The IGF-1R is frequently upregulated in cancers and signals from the cell surface to
Katrin Friedbichler et al.
Molecular cancer therapeutics, 13(2), 399-409 (2013-12-04)
Insulin-like growth factor (IGF) signaling is thought to play a role in the development and progression of multiple cancer types. To date, therapeutic strategies aimed at disrupting IGF signaling have largely focused on antibodies that target the IGF-I receptor (IGF-IR).
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