A specific chromogenic substrate for human neutrophil elastase (Kcat/Km = 120,000 vs. 2700 for pancreatic elastase). Cleavage can be monitored at ~410 nm. Sold on the basis of substrate content.
Specific chromogenic substrate for human neutrophil elastase (kcat/Km = 120,000 versus pancreatic elastase: kcat/Km = 2,700). Cleavage can be monitored at 410 nm using ε = 8,800.
Biochem/physiol Actions
Cell permeable: no
Kcat/Km = 120,000 vs. 2700 for pancreatic elastase
Primary Target Chromogenic substrate for human neutrophil elastase
Product does not compete with ATP.
Reversible: no
Warning
Toxicity: Standard Handling (A)
Sequence
MeOSuc-Ala-Ala-Pro-Val-pNA
Physical form
Lyophilized from a solution containing 90 mg D-mannitol and 10 mg substrate.
Reconstitution
Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Other Notes
Barrett, A.J., et al. 1989. Biochim. J.260, 259. Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
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Elastase is a globular glycoprotein and belongs to the chymotrypsin family. It is involved in several inflammatory cascades on the basis of cleaving the important connective tissue protein elastin, and is strictly regulated to a balance by several endogenous inhibitors.
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