Skip to Content
Merck
All Photos(1)

Documents

M9771

Sigma-Aldrich

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin

elastase substrate

Synonym(s):

MeOSuc-AAPV-AMC

Sign Into View Organizational & Contract Pricing
All Photos(1)

About This Item

Empirical Formula (Hill Notation):
C31H41N5O9
CAS Number:
72252-90-5
Molecular Weight:
627.69
MDL number:
MFCD00077135
UNSPSC Code:
12352204
PubChem Substance ID:
24897367
NACRES:
NA.32

Assay

≥98% (HPLC)

form

powder

solubility

chloroform: 50 mg/mL, clear, colorless

storage temp.

−20°C

SMILES string

COC(=O)CCC(=O)NC(C)C(=O)NC(C)C(=O)N1CCCC1C(=O)NC(C(C)C)C(=O)Nc2ccc3C(C)=CC(=O)Oc3c2

InChI

1S/C31H41N5O9/c1-16(2)27(30(42)34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,34,42)(H,35,41)

InChI key

CMEUDEVBFFPSEI-UHFFFAOYSA-N

Application

N-Methoxysuccinyl-Ala-Ala-Pro-Val-7-amido-4-methylcoumarin has been used as a substrate in elastase assay using bone marrow lysates and pancreatic lysates.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Substrates

Fluorogenic substrate for the assay of human leukocyte and porcine pancreatic elastase.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Gerrit Wolters-Eisfeld et al.
Experimental & molecular medicine, 50(10), 1-13 (2018-10-12)
Cosmc is ubiquitously expressed and acts as a specific molecular chaperone assisting the folding and stability of core 1 synthase. Thus, it plays a crucial role in the biosynthesis of O-linked glycosylation of proteins. Here, we show that ablation of
Jonas Hahn et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(1), 1401-1414 (2018-08-22)
Papillon-Lefèvre syndrome (PLS) is characterized by nonfunctional neutrophil serine proteases (NSPs) and fulminant periodontal inflammation of unknown cause. Here we investigated neutrophil extracellular trap (NET)-associated aggregation and cytokine/chemokine-release/degradation by normal and NSP-deficient human and mouse granulocytes. Stimulated with solid or
Sensitive substrates for human leukocyte and porcine pancreatic elastase: a study of the merits of various chromophoric and fluorogenic leaving groups in assays for serine proteases.
M J Castillo et al.
Analytical biochemistry, 99(1), 53-64 (1979-10-15)
D Polacek et al.
The Journal of biological chemistry, 261(5), 2057-2063 (1986-02-15)
In vitro incubation of human plasma low density lipoproteins (LDL) with human blood polymorphonuclear cells (PMN) for 1 h at 37 degrees C resulted in an increased (2-4-fold) release into the medium of an enzymatic activity which co-eluted with LDL
Gisele Pena de Oliveira et al.
Nutrients, 11(4) (2019-04-25)
The innate immune response plays an important role in the pathophysiology of acute respiratory distress syndrome (ARDS). Glutamine (Gln) decreases lung inflammation in experimental ARDS, but its impact on the formation of extracellular traps (ETs) in the lung is unknown.
View All Related Papers

Articles

Elastase Function and Importance

Elastase application index for understanding leukocyte elastase, a 29KDa serine endoprotease.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service