CRISPR Cas9 Proteins & Gene Editing
THE ULTIMATE SUITE OF CRISPR CAS9 PROTEIN ESSENTIALS AND SERVICES
Advances in genome engineering are enabling researchers to achieve unprecedented breakthroughs, from curing genetic disorders to developing disease-resistant crops. Our reliable CRISPR Cas9 protein essentials and tools deliver complete support for every step of CRISPR-based gene editing research from bench-top to larger scales. Target a single gene using Zinc Finger Nucleases or screen a whole genome with fully customizable CRISPR products.
For incremental innovations, day after day, choose CRISPR Cas9 proteins and gene editing tools that are the foundation of great work, not a variable that undermines it.
Section Overview
The Sigma-Aldrich® Advanced Genomics portfolio delivers the ultimate suite of genome engineering tools and services to unlock your research potential. Whether you are looking to knockout, knock-in, knockdown, or overexpress your targets, our comprehensive suite of CRISPR gene-editing tools and services will take your research Beyond the Bench.
Best in Class Cas9 Proteins
Sigma-Aldrich® Advanced Genomics offers multiple Cas9 variants (wild-type, enhanced specificity, nickase, GFP-fused, catalytically inactive) and in several formats (plasmid, lentivirus, lyophilized protein). Choose from an industry leading selection of Cas9 proteins to meet your individual research needs.
- Explore our full list of Cas9 Products
- Learn more about Cas9-GFP Fusion Proteins
Guaranteed CRISPR gRNA
Every SygRNA® synthetic single guide RNA (sgRNA), crRNA and tracrRNA is synthesized using our proprietary process and reagents using the highest quality materials available. Editing activity is assured regardless of your target, cell type or application, and is backed by the most comprehensive guarantee in the industry.
- Order pre-designed synthetic CRISPR guide RNA (sgRNA)
- Order custom synthetic CRISPR RNA
- Learn more about PURedit™ RNP reagents
CRISPR Inhibition and Activation (CRISPRi and CRISPRa)
Gain even greater insights into complex biological networks using Sigma-Aldrich® Advanced Genomics CRISPR activation and CRISPR inhibition libraries for gene knockdown and overexpression experiments. Pooled CRISPRi and SAM CRISPRa lentiviral libraries are available off-the-shelf or customized to your specific needs.
CRISPR Screening with 10x Genomics Compatibility
Sigma-Aldrich® Advanced Genomics offers the only 10x Genomics Certified Compatible lentiviral pools for CRISPR single-cell screening. Our optimized, custom lentiviral vectors are fully compatible with Chromium Single Cell Gene Expression v3 and v3.1 (Next GEM) solution utilizing Feature Barcode technology.
We’re Here to Help
Whether you need help designing your CRISPR experiment, require assistance troubleshooting, or simply want to better understand how you can apply gene editing methods in your research, Sigma-Aldrich® Advanced Genomics is here to guide you.
Wondering if CRISPR is right for your project? Connect with a Sigma-Aldrich® Advanced Genomics Expert.
Related Product Resources
- Article: Cas9-Mediated Recombineering Enhances Metabolic Production of Kdo2-Lipid A by E. coli
In this article, we present an application of our novel E. coli CRISPR/Cas-mediated Lambda-Red (λ-Red) homologous recombination (HR) vector system, which facilitates gene editing through the homology-directed repair (HDR) of double-stranded DNA breaks (DSBs) created by Cas9 endonuclease, using either ssDNA or dsDNA as an editing template.
- CRISPR Use License Agreement
This Product and its use are the subject of one or more of the following patents controlled by The Broad Institute, Inc. (BROAD), the Massachusetts Institute of Technology (MIT), or the President and Fellows of Harvard College (HARVARD): U.S. Patent Nos. 8,697,357; 8,771,945, and any substitutions, divisions, continuations, reissues, renewals, re-examinations or extensions, and corresponding foreign patent applications and patents.
- Article: Ribonucleoprotein (RNP) Protocols Using Synthetic sgRNAs and Cas9 proteins
Combine guaranteed sgRNAs with our comprehensive range of CRISPR products and tools, including Cas9 and delivery reagents, for efficient genome modification with higher specificity.
- Article: Genome Editing in Plants with CRISPR/Cas9
Zinc finger nucleases (ZFNs), were introduced by Sigma-Aldrich less than 8 years ago, but in that time the technology of targeted genome editing has advanced rapidly. Most recently, the discovery of the CRISPR/Cas9 pathway has accelerated interest in this field, opening up new possibilities for research and development.
- eBook: Gene Editing 101
Learn how to understand, design and perform CRISPR experiments, as well as design gRNA, choose a Cas9 format, screen with CRISPR, use advanced CRISPR formats, and much more…
- Article: CRISPR Cas9 Protein Product Selection Guide
A convenient CRISPR Cas9 protein selection guide to assist with your CRISPR RNP, lentivirus, mRNA, plasmids, vectors and control needs.
- Article: Blue-White Screening & Protocols for Colony Selection
Our popular blue-white screening for colony selection technical resource hub, transfection reagent selection guide and protocols.
- PCR Master Mix Calculator
Ensure the success of your reactions at any scale with our handy PCR Master Mix Calculator resource tool.
- Article: Introduction to Cell Transfection
An overview on cell transfection vs transduction methods, our cell transfection reagent selection guide and protocols.
- Protocol: Western Blotting
Western blotting protocol that includes technical information supporting the following: Sample preparation, membrane transfer/tank transfer, transfer stack assembly, buffers, immunodetection, and a troubleshooting guide.
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