ZRB1683

Anti-HSL/LIPE Antibody, clone 1D5 ZooMAb^® Rabbit Monoclonal

recombinant, expressed in HEK 293 cells

• Synonym(s): Hormone-sensitive lipase
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 200
• recombinant: expressed in HEK 293 cells
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1D5, recombinant monoclonal
• description: recombinant, expressed in HEK 293 cells
• product line: ZooMAb^® learn more
• form: lyophilized
• mol wt: calculated mol wt 116.6 kDa; observed mol wt ~75 kDa
• purified by: using Protein A
• species reactivity: mouse, human
• packaging: antibody small pack of 25 μL
• greener alternative product characteristics: Waste Prevention; Designing Safer Chemicals; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• enhanced validation: recombinant expression; Learn more about Antibody Enhanced Validation
• sustainability: Greener Alternative Product
• technique(s): immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; western blot: suitable
• isotype: IgG
• epitope sequence: C-terminal half
• Protein ID accession no.: NP_005348
• UniProt accession no.: Q05469
• greener alternative category: , Aligned
• shipped in: ambient
• storage temp.: 2-8°C
• target post-translational modification: unmodified
• Gene Information: human ... LIPE(3991)

Description

General description

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.

ZooMAb^® antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb^® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb^® antibodies are reliably available and ready to ship when you need them.

Specificity

Clone 1D5 is a ZooMAb^® Rabbit recombinant monoclonal antibody that specifically detects Hormone-sensitive lipase (HSL). It targets an epitope within 23 amino acids from the C-terminal half.

Immunogen

KLH-conjugated linear peptide corresponding to 23 amino acids from the C-terminal half of human Hormone sensitive lipase (HSL/LIPE).

Application

Quality Control Testing

Evaluated by Western Blotting in HepG2 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected HSL/LIPE in HepG2 cell lysate.

Tested applications

Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected HSL/LIPE in MCF7 and NIH3T3 cell lysates.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected HSL/LIPE in human testis tissue sections.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected HSL/LIPE in A431 cells..

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user

Target description

Hormone-sensitive lipase (UniProt: Q05469; also known as EC:3.1.1.79, HSL, Monoacylglycerol lipase, LIPE, Retinyl ester hydrolase, REH) is encoded by the LIPE gene (Gene ID: 3991) in human. HSL is an intracellular, neutral lipase with broad substrate specificity. It is known to exist as a monomer or as a homodimer. HSL is found in the high-density caveolae and it translocates to the cytoplasm from the caveolae upon insulin stimulation. It catalyzes the hydrolysis of triacylglycerols (TAGs), diacylglycerols (DAGs), monoacylglycerols (MAGs), cholesteryl esters, and retinyl esters. However, it does not possess phospholipase activity. It shows a preferential hydrolysis of DAGs over TAGs and MAGs and preferentially hydrolyzes the fatty acid (FA) esters at the sn-3 position of the glycerol backbone in DAGs. Its activity against DAGs is shown to be about 10-fold and 5-fold higher than its activity against TAGs and MAGs, respectively. Its hydrolytic activity against TAGs and cholesteryl esters, but not against DAGs, is stimulated by phosphorylation mediated primarily by protein kinase A. Its phosphorylation by AMPK is reported to reduce its translocation towards the lipid droplets. HSL -/- mice are shown to be resistant to obesity. Mutations in LIPE gene are known to cause familial lipodystrophy that is characterized by abnormal subcutaneous fat distribution and affected subjects show increased visceral fat, impaired lipolysis, dyslipidemia, and hepatic steatosis, and systemic insulin resistance. This ZooMAb^® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Rodriguez, JA., et al. (2010). Biochim. Biophys. Acta. 1801(1); 77-83; Aboulaich N., et al. (2006). Biochem. Biophys. Res. Commun. 350(3); 657-661; Kraemer, FB., and Shen, WJ (2006). Nutr. Metab. 3; Article 12).

Physical form

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS, 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

Reconstitution

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

Legal Information

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable