T6424

Trypsin from human pancreas

salt-free, lyophilized powder, vial of ≥1,000 BAEE units

• Synonym(s): Serine Protease 1
• CAS Number: 9002-07-7
• Enzyme Commission number: 3.4.21.4 (BRENDA, IUBMB)
• EC Number: 232-650-8
• MDL number: MFCD01323069
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• biological source: human pancreas
• Quality Level: 200
• form: salt-free, lyophilized powder
• packaging: vial of ≥1,000 BAEE units
• UniProt accession no.: P07477; P07478; P35030
• storage temp.: −20°C
• Gene Information: human ... PRSS1(5644) , PRSS2(5645) , PRSS3(5646)

Description

General description

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

Trypsin is a serine protease. The pancreas produce the precursor trypsinogen and secretes it to the intestine. Trypsinogen is converted to trypsin by enterokinase in the intestinal mucous membrane.

Application

Trypsin has been used in a study to assess the similarities between the hepatitis E virus and human astrovirus. Trypsin has also been used in a study to characterize a unique technique for culturing primary adult human epithelial progenitor, or stem, cells.

Unit Definition

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25 °C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

Quantity

Minimum 1,000 BAEE units/vial

Analysis Note

Protein determined by E1%/280.

Other Notes

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 1
• ppe: dust mask type N95 (US), Eyeshields, Faceshields, Gloves