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Key Documents

SRP6549

Sigma-Aldrich

Alkaline Phosphatase from calf intestine

≥98% (SDS-PAGE)

Synonym(s):

ALP, ALP-1, ALPP, ALPPL, ALPPL2, PALP, PLAP, PLAP-1

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About This Item

EC Number:
232-631-4
UNSPSC Code:
12352205

biological source

bovine (calf) intestine

assay

≥98% (SDS-PAGE)

form

freeze-dried protein glass

packaging

pkg of 1 mg
pkg of 10 μg
pkg of 50 μg

impurities

3000-6,000 EU/μg endotoxin (LAL test)

UniProt accession no.

P09487

shipped in

wet ice

storage temp.

−20°C

Gene Information

bovine (calf) ... ALPL(280994)

General description

Alkaline phosphatase (ALP) is a hydrolase enzyme. It is a highly active form in calf intestinal mucosa.[1][2] The calf intestinal alkaline phosphatase is a dimeric glycoprotein with a molecular weight of about 140kDa.[3]

Application

Alkaline phosphatase from calf intestine has been used as a standard in alkaline phosphatase assay.[4]

Biochem/physiol Actions

Alkaline phosphatase (ALP) is responsible for removing phosphate groups at the 5- and 3- positions from many types of molecules, such as nucleotides, proteins, and alkaloids.[2] It can also modify lipopolysaccharides by dephosphorylation of the lipid A moiety, thereby making it less toxic than the unmodified lipid A.[5]

Physical form

Lyophilized without any additives.

Reconstitution

Reconstitute in distilled water or dilute buffer.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
5C04L75850
9D147585

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Affinity-chromatography purification of alkaline phosphatase from calf intestine.
Brenna O
The Biochemical Journal, 151, 291-296 (1975)
Quantitation of surface coverage of oligonucleotides bound to chip surfaces: a fluorescence-based approach using alkaline phosphatase digestion.
Pal S
Lab on a chip, 8, 1332-1341 (2008)
Pyrophosphate inhibits mineralization of osteoblast cultures by binding to mineral, up-regulating osteopontin, and inhibiting alkaline phosphatase activity.
Addison WN
The Journal of Biological Chemistry, 282, 15872-15883 (2007)
Intestinal alkaline phosphatase detoxifies lipopolysaccharide and prevents inflammation in zebrafish in response to the gut microbiota.
Cell host & microbe, 2, 371-382 (2007)
Intestinal alkaline phosphatase. Physical properties and quaternary structure.
M Fosset et al.
Biochemistry, 13(9), 1783-1788 (1974-04-23)

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