SCJ4600015

CF^™ 488A hydrazide

suitable for fluorescence, ≥90% (HPLC)

• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• assay: ≥90% (HPLC)
• form: solid
• mol wt: ~928 g/mol
• solubility: H₂O: soluble
• ε (extinction coefficient): 70000 at 490 nm
• absorbance ratio: A_{280/490 nm} 0.1
• fluorescence: λ_ex 488 nm (Flow cytometry laser line); λ_ex 488 nm (Microscopy laser line); λ_ex 490 nm; λ_em 515 nm
• suitability: suitable for fluorescence
• storage temp.: −20°C

Description

General description

CF^™488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection conditions, CF^™488A is at least as bright as Alexa Fluor^™ 488. However, a major advantage of CF^™488A over Alexa Fluor^™ 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor^™ 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF^™488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF^™488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor^™ 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF^™488A offers the advantage of less fluorescence "spill-over" in the red channel in multi-color detection applications.

CF^™ dye hydrazides can be used as a fixable polar tracers for visualizing cell morphology,or for gap junction studies. The dyes can be introduced into cells by microinjection. For conjugation to aldehyde or ketone groups (such as carbohydrate molecules after peroxidation with periodate) we recommend using the more reactive CF aminooxy dye forms.

Features and Benefits

1. Yields biologically more specific antibody conjugates and has less "spill-over" fluorescence in the red channel than Alexa Fluor^™488. 2. Extremely photostable. 3. Highly water-soluble and pH-insensitive.

Preparation Note

Protect from light

Reconstitution

Dissolve in water

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.

3D Insert is a trademark of 3D Biotek, LLC

ALEXA FLUOR is a trademark of Life Technologies

CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable