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N7885

Sigma-Aldrich

Neuraminidase from Vibrio cholerae

Type III, buffered aqueous solution, 0.2 μm filtered, 1-5 units/mg protein (Lowry, using NAN-lactose)

Synonym(s):

Acyl-neuraminyl Hydrolase, Receptor-destroying enzyme, Sialidase

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1 UNIT
$187.00
2 UNITS
$319.77

$187.00

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1 UNIT
$187.00
2 UNITS
$319.77

About This Item

CAS Number:
9001-67-6
Enzyme Commission number:
3.2.1.18 (BRENDA, IUBMB)
EC Number:
232-624-6
MDL number:
MFCD00131711
UNSPSC Code:
12352204
NACRES:
NA.54

$187.00

In StockDetails

Request a Bulk Order

Quality Level

300

type

Type III

form

buffered aqueous solution

specific activity

1-5 units/mg protein (Lowry, using NAN-lactose)

mol wt

83 kDa

application(s)

diagnostic assay manufacturing

foreign activity

Protease and NAN-aldolase, present

storage temp.

2-8°C

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General description

Neuraminidase enzymes are glycoside hydrolase enzymes that catalyze hydrolysis of terminal sialic acid residues. The most well-known are the viral nearamidases, which promote influenza virus release.

Application

Neuraminidase from Vibrio cholerae has been used in a study to assess its role in the binding and uptake of cholera toxin by susceptible cells. [1] It has also been used in a study to investigate the preparation of a sodium substrate and its use in a fluorometric assay of neuraminidase. [2]
Neurminidase is used as a cell-surface probe for glycoconjugate distribution and in substrate specificity studies.

Biochem/physiol Actions

Vibrio cholerae neuraminidase has been shown to promote cholera toxin infection by binding the toxin and aiding in its uptake by cells. [1]

Quality

Preservative free.

Unit Definition

One unit will liberate 1.0 μmole of N-acetylneuraminic acid per min at pH 5.0 at 37 °C using NAN-lactose or bovine submaxillary mucin, unless otherwise specified. Prices based on units using NAN-lactose as substrate.

Physical form

Aqueous solution, pH 5.5, containing 0.15 M NaCl and 4 mM CaCl2.

Preparation Note

Chromatographically purified.

pictograms

Health hazard
GHS08

signalword

Danger

hcodes

H334

Hazard Classifications

Resp. Sens. 1

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
0000302547
0000167386
0000232683
0000256064
0000256063

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Paola Rota et al.
Organic & biomolecular chemistry, 10(14), 2885-2894 (2012-03-08)
A simple protocol for the synthesis of N-perfluoroacylated and N-acylated glycals of neuraminic acid, with a secondary cyclic amine (morpholine or piperidine) at the 4α position, has been set-up, starting from peracetylated N-acetylneuraminic acid methyl ester that undergoes, sequentially to
Jai Woong Seo et al.
Nature communications, 11(1), 2102-2102 (2020-05-02)
Adeno-associated viruses (AAVs) are typically single-stranded deoxyribonucleic acid (ssDNA) encapsulated within 25-nm protein capsids. Recently, tissue-specific AAV capsids (e.g. PHP.eB) have been shown to enhance brain delivery in rodents via the LY6A receptor on brain endothelial cells. Here, we create
P C Reading et al.
Journal of virology, 74(11), 5190-5197 (2000-05-09)
Influenza viruses A/PR/8/34 (PR8; H1N1), A/Aichi/68 X-31 (HKx31; H3N2), and A/Beijing/89 X-109 (BJx109; H3N2) show marked differences in their ability to infect murine macrophages, including resident alveolar and peritoneal macrophages as well as the macrophage-derived cell line J774. The hierarchy
Fluorometric assay of neuraminidase with a sodium (4-methylumbelliferyl-alpha-D-N-acetylneuraminate) substrate.
M Potier et al.
Analytical biochemistry, 94(2), 287-296 (1979-04-15)
J E Galen et al.
Infection and immunity, 60(2), 406-415 (1992-02-01)
Vibrio cholerae neuraminidase (NANase) is hypothesized to act synergistically with cholera toxin (CT) and increase the severity of a secretory response by increasing the binding and penetration of CT to enterocytes. To test this hypothesis, the NANase gene (nanH) from
View All Related Papers

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