MAK290
Trypsin Activity Colorimetric Assay Kit
sufficient for 100 colorimetric tests
Synonym(s):
Trypsin Enzyme Activity Kit
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About This Item
UNSPSC Code:
12161503
NACRES:
NA.84
Recommended Products
detection method
colorimetric
relevant disease(s)
gastrointestinal diseases
storage temp.
−20°C
General description
Trypsin (EC 3.4.21.4) is a pancreatic serine protease, which hydrolyzes peptide bonds specifically at the carboxyl side of arginine and lysine residues. The rate of hydrolysis is slower if an acidic residue is on either side of the cleavage site and cleavage may not occur if a proline residue is on the carboxyl side. Tryptic digestion of the protein of interest results in a highly specific cleavage and a limited number of peptide fragments.
Suitability
Suitable for the detection of Trypsin activity in various samples.
Principle
In this assay, trypsin cleaves a substrate to generate p-nitroaniline (p-NA) which is detected at λ= 405 nm. Since the color intensity is proportional to p-NA content, trypsin activity can be accurately measured. The kit detects 10–100 mU (p-NA unit) trypsin in various samples.
signalword
Danger
hcodes
Hazard Classifications
Eye Dam. 1 - Resp. Sens. 1
Storage Class
10 - Combustible liquids
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Protocols
Continuous spectrophotometric rate determination method using BAEE substrate measures trypsin activity, essential for enzyme characterization.
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