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H2625

Sigma-Aldrich

Hemoglobin from bovine blood

suitable for protease substrate, substrate powder

Synonym(s):

Bovine hemoglobin, Hb, Methemoglobin

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About This Item

CAS Number:
9008-02-0
MDL number:
MFCD00131282
UNSPSC Code:
12352202
eCl@ss:
42030116
NACRES:
NA.61

biological source

bovine blood

form

substrate powder

mol wt

Mr ~64500

technique(s)

activity assay: suitable

solubility

H2O: soluble 20 mg/mL

suitability

suitable for protease substrate

UniProt accession no.

P01966
P02070
P02081
P06642
P06643

storage temp.

2-8°C

Gene Information

cow ... HBA1(281221) , HBB(280813) , HBE2(513240) , HBE4(513108) , HBG(511735) , alpha globin(512439)

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General description

Hemoglobin is the major component of red blood cells and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is a globular protein with α and β chains with every 141 and 146 amino acids respectively. It exists as a tetramer with each monomer having heterocyclic porphyrin ring with iron constituting the heme.
Hemoglobin is the major component of red blood cells, and is responsible for their red color. Its normal concentration in erythrocytes is 34%. Hemoglobin is the most important respiratory protein of vertebrates by virtue of its ability to transport oxygen from the lungs to body tissues, and to facilitate the return transport of carbon dioxide.

Application

Hemoglobin from bovine blood has been used as a substrate in cathepsin D activity assay. It has also been used to measure the activity of acid proteases (pepsin-like) in the stomach extract.
The solubility of α-elastin has been applied to construction of elastin-mimetic biomaterials.

Biochem/physiol Actions

Bovine hemoglobin is used in the production of hemoglobin-vesicles (HbV). It has high thermal stability and high oxygen affinity when compared to human hemoglobin. Bovine hemoglobin interacts with synthetic and azo dyes. Polymerized forms of bovine hemoglobin are used to treat autoimmune hemolytic anemia.
Oxygen transporter. The Fe2+/Fe3+ balance is a physiological indicator of blood oxygenation; deoxygenated hemoglobin accessorizes a feedback loop by reducing nitrite to NO, a vasodilator which enhances blood flow to oxygen-deprived tissues.

Caution

Since native hemoglobin is readily oxidized in air, these preparations may be predominantly methemoglobin.

Preparation Note

Prepared from washed, lysed and dialyzed erythrocytes.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Luke J Norbury et al.
Biochimie, 93(3), 604-611 (2010-12-21)
Cathepsin proteases are promising vaccine or drug targets for prophylaxis or therapy against Fasciola parasites which express cathepsin L and B proteases during their development. These proteases are believed to be involved in important functions for the parasite, including excystment
A study of the interaction of bovine hemoglobin with synthetic dyes using spectroscopic techniques and molecular docking
Bansal S, et al.
Frontiers in Chemistry, 4(4), 50-50 (2017)
Xianbo Lu et al.
Biosensors & bioelectronics, 23(8), 1236-1243 (2007-12-18)
Carbon nanofibers (CNFs), with typical diameters of approximately 80 nm and lengths of the order of micrometers, are extremely attractive in bioanalytical area as they can combine properties of high surface area, non-toxicity, acceptable biocompatibility, ease of fabrication, chemical and
Characteristics of bovine hemoglobin as a potential source of hemoglobin-vesicles for an artificial oxygen carrier
Sakai H, et al.
Journal of Biochemistry, 131(4), 611-617 (2002)
Circadian cycle of digestive enzyme production at fasting and feeding conditions in Nile tilapia, Oreochromis niloticus (Actinopterygii: Perciformes: Cichlidae)
MEJIA, MAGNOLIA MONTOYA and others
Acta ichthyologica et piscatoria, 32(2), 188-193 (2016)
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Protocols

Enzymatic Assay of Pepsin (3.4.23.1)

This procedure may be used for determination of Pepsin activity using hemoglobin as the substrate. It is a spectrophotometric stop rate determination.

Proteinase K Enzymatic Assay

Proteinase K activity measured via spectrophotometry using hemoglobin substrate, crucial for enzyme characterization.

Chromatograms

application for HPLC

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