GE17-0978-02

Butyl-s Sepharose^™ 6 Fast Flow

Cytiva 17-0978-02, pack of 200 mL

• UNSPSC Code: 41106500
• NACRES: NA.56

Properties

• ligand: butyl-S
• packaging: pack of 200 mL
• manufacturer/tradename: Cytiva 17-0978-02
• matrix: 6% cross-linked agarose
• particle size: 45-165 μm
• average diameter: 90 μm
• cleaning in place: 2-14
• working range: 3-13
• suitability: suitable for bioprocess medium

Description

General description

Butyl-S Sepharose^™ 6 Fast FLow is the least hydrophobic medium in the Sepharose^™ Fast FLow hydrophobic interaction chromatography (HIC) range and is particularly useful during the initial stages of a purification process to remove the bulk of impurities.

Application

Butyl-S Sepharose^™ 6 Fast FLow is part of the Sepharose^™ Fast FLow HIC platform, which has been an industrial standard for HIC chromatography during recent decades. Butyl-S Sepharose^™ 6 Fast FLow is composed of cross-linked 6% Agarose beads modified with butyl groups via a thio-ether linkage. It will exhibit a different selectivity compared to Butyl Sepharose^™ 4 Fast FLow and other HIC Sepharose^™ Fast FLow products.

This resin is designed for the binding and elution of relatively strong hydrophobic molecules at comparatively Low salt concentrations. Butyl?S Sepharose^™ 6 Fast FLow is particularly useful during the initial stages of a separation process to remove the bulk of impurities without stringent requirements for conditioning of the sample (e.g. adjustment of pH, salt concentration etc.)

Butyl-S Sepharose^™ 6 Fast FLow is available in a range of different bulk pack sizes and convenient pre-packed formats for easy scale-up and process development. As member of the BioProcess media range, Butyl-S Sepharose^™ 6 Fast FLow meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

• Binds and elutes relatively strong hydrophobic molecules at comparatively Low salt concentrations
• The least hydrophobic medium in the well proven Sepharose^™ Fast FLow HIC range
• Purifies recombinant Hepatitis B virus surface antigen from CHO cells
• Developed and optimized in cooperation with a process-scale manufacturer of biopharmaceuticals
• The hydrophilic nature of the base matrix ensures Low levels of non-specific binding.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

Sepharose is a trademark of Cytiva

Safety Information

• pictograms: GHS02
• signalword: Warning
• hcodes: H226
• pcodes: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Storage Class: 3 - Flammable liquids