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G9785

Sigma-Aldrich

GSEM Supplement

liquid, suitable for cell culture,

Synonym(s):

Glutamine synthetase expression medium supplement

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100 ML
$132.00

$132.00

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100 ML
$132.00

About This Item

MDL number:
MFCD00677096
UNSPSC Code:
12352205
NACRES:
NA.75

$132.00

Check Cart for Availability
Request a Bulk Order

Product Name

GSEM Supplement, 50 ×, liquid, sterile-filtered, suitable for cell culture

sterility

sterile-filtered

Quality Level

200

form

liquid

concentration

50 ×

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

dry ice

storage temp.

−20°C

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General description

Glutamine synthetase expression medium (GSEM) supplement enables formation of glutamine from ammonia and glutamate there by alleviating accumulation of ammonia in culture medium.[1] It is exploited for high yield recombinant protein production in Chinese hamster ovary (CHO) cells.[2]

Application

GSEM Supplement has been used as a supplement for ProCHO5 Protein-free medium for culturing Chinese hamster ovary (CHO) cells[3] and selective medium for NS0 myeloma cells.[4]

Packaging

Contains (mg/L): L-alanine 450.0, L-asparagine · H2O 4261.0, L-aspartic acid 650.0, L-glutamic acid 3750.0, L-proline 575.0, L-serine 500.0, adenosine 350.0, guanosine 350.0, cytidine 350.0, uridine 350.0, and thymidine 12.0

Quantity

One mL will supplement 50 mL of cell culture medium.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
RNBK6922
RNBK0947
RNBJ6154
RNBJ1346
RNBH2789

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Comprehensive characterization of glutamine synthetase-mediated selection for the establishment of recombinant CHO cells producing monoclonal antibodies
Noh SM, et al.
Scientific reports, 8(1), 5361-5361 (2018)
Soluble Fc fusion proteins for biomedical research
Monoclonal Antibodies: Methods and Protocols, 33-52 (2007)
A streamlined implementation of the glutamine synthetase-based protein expression system
Knox R, et al.
BMC biotechnology, 13(1), 74-74 (2013)
Novel membrane?bound reporter molecule for sorting high producer cells by flow cytometry.
Helman D, et al.
Cytometry. Part A : the Journal of the International Society For Analytical Cytology, 85(2), 162-168 (2014)
Doyle, A., et al.
Cell and Tissue Culture: Laboratory Procedures, 27D, 3-3 (1999)
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