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Detroit 573

90112601, human skin, Fibroblast-like

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90112601-DNA-5UG
$402.00
1 VIAL
$847.00

$402.00


Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery


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90112601-DNA-5UG
$402.00
1 VIAL
$847.00

About This Item

UNSPSC Code:
41106514

$402.00


Usually ships in 1 week. (Orders outside of Europe & Scandinavian countries, allow an additional week for delivery

Product Name

Detroit 573, 90112601, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

biological source

human skin

packaging

tube of 5 μg 90112601-DNA-5UG
pkg of vial of cells 90112601-1VL

growth mode

Adherent

karyotype

Aneuploid

morphology

Fibroblast-like

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human Caucasian skin, B/D translocation

Cell Line Description

Detroit 573 was initiated in 1967 from a skin biopsy of a deceased new-born female with multiple congenital anomalies. Approximately 94% of the cell population is 45,XX,t(Bp-;Dq+). The translocated B/D chromosome morphologically resembles a group A-2 chromosome. The cell line is susceptible to polyvirus type 1 and vesicular stomatitis (Indiana Strain) virus.

Application

Karyology studies

Culture Medium

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

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