Antifoam A is a silicone-based product used to prevent or minimize foaming in both microbial fermentation systems and mammalian suspension cultures. Control of foaming minimizes damage to the cells, resulting in increased protein/antibody production. Extremely effective for aqueous and non-aqueous systems. Effective at concentrations in the 1-50ppm range.
Application
Antifoam A concentrate has been used:
in bioreactor culture for Staphylococcal enterotoxin C (SEC) production.[1]
to prevent foam formation during the aerobic treatment of sludge. [2]
to study the impact of surface tension on bioaerosol generation during bubbling. [3]
Features and Benefits
Molecular biology grade and tested for use in bacterial fermentation.
Extremely effective foam suppressor for aqueous and non-aqueous systems.
Made of 100% active silicone polymer, free of emulsifiers.
Typically, effective at 1-100 ppm.
Product will be stable in the pH range of 5 to 9.
Can be directly added to a fermentation medium but it is not recommended that it be pumped to a fermenter on an as-needed basis.
Components
Antifoam A is a 100% active silicon polymer containing no non-ionic emulsifiers .
Preparation Note
Antifoam A can be pre-diluted with 3-10 parts of aqueous propylene glycol or non-aqueous vegetable oil with slow mixing.
Journal of bacteriology, 172(9), 5119-5129 (1990-09-01)
Enzymatic conversion of 4-fluorocatechol in the simultaneous presence of partially purified preparations of catechol 1,2-dioxygenase from Pseudomonas cepacia and muconate cycloisomerase from Alcaligenes eutrophus 335 yielded a product that was unambiguously identified as (+)-4-fluoromuconolactone [(+)-4-carboxymethyl-4-fluoro-but-2-en-4-olide]. This compound was shown to
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