398187

Xylenol Orange tetrasodium salt

ACS reagent

• Synonym(s): 3,3′-Bis[N,N-bis(carboxymethyl)aminomethyl]-o-cresolsulfonephthalein tetrasodium salt
• Empirical Formula (Hill Notation): C₃₁H₂₈N₂Na₄O₁₃S
• CAS Number: 3618-43-7
• Molecular Weight: 760.58
• EC Number: 222-805-8
• MDL number: MFCD00044293
• UNSPSC Code: 12171500
• PubChem Substance ID: 24864725
• NACRES: NA.47

Properties

• grade: ACS reagent
• Quality Level: 200
• form: powder or crystals
• color: very dark brown to very dark red
• mp: 195 °C (dec.) (lit.)
• λmax: 580 nm
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: [Na+].[Na+].[Na+].[Na+].Cc1cc(cc(CN(CC([O-])=O)CC([O-])=O)c1O)C2(OS(=O)(=O)c3ccccc23)c4cc(C)c(O)c(CN(CC([O-])=O)CC([O-])=O)c4
• InChI: 1S/C31H32N2O13S.4Na/c1-17-7-21(9-19(29(17)42)11-32(13-25(34)35)14-26(36)37)31(23-5-3-4-6-24(23)47(44,45)46-31)22-8-18(2)30(43)20(10-22)12-33(15-27(38)39)16-28(40)41;;;;/h3-10,42-43H,11-16H2,1-2H3,(H,34,35)(H,36,37)(H,38,39)(H,40,41);;;;/q;4*+1/p-4
• InChI key: YIIQUYHVWRJXEM-UHFFFAOYSA-J

Description

General description

Xylenol Orange tetrasodium salt is a fluorochrome acid dye with complexing and chelating properties that make it useful as an indicator in the spectrophotometric detection and assay of metal ions such as calcium, cadmium, bismuth, zinc, lead, and mercury. Its tetrasodium salt especially is used as an indicator in metal titrations. It changes color from yellow to red between pH 6.4 to 10.4. It has six potentially anionic substituents, one being sulfonate, and is anionic under staining conditions and during most laboratory applications.

Application

• Xylenol Orange has been used as a vital stain to study calcification in bone development, injury, and repair.
• It has been used in the fluorescent histochemical detection of alkaline phosphatase.
• It has been used in FBX (ferrous benzoic xylenol orange) gel preparation to study the effects of MRI (magnetic resonance imaging) parameters on image quality.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, type N95 (US)