MABD20

Anti-PAX7 Antibody, clone 2F12H4

clone 2F12H4, from rat

• Synonym(s): paired box 7, PAX7 transcriptional factor, paired box gene 7, paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rat
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: 2F12H4, monoclonal
• species reactivity: mouse, human (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039)
• technique(s): ChIP: suitable; immunocytochemistry: suitable; western blot: suitable
• isotype: IgG2bκ
• NCBI accession no.: NP_035169
• UniProt accession no.: P47239
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Pax7(18509)

Description

General description

Paired box protein Pax-7 (PAX7) belongs to the Pax family of transcription factors, a highly conserved group of proteins with crucial roles in tissue homeostasis and organogenesis. PAX7 is involved in the specification, survival, maintenance, and self-renewal of skeletal muscle progenitors and myogenic progenitors. It is primarily expressed in newly activated and quiescent satellite cells. Studies have shown that on activation, quiescent satellite cells co-express MyoD with PAX7 prior to proliferation, after which one or the other transcription factor is downregulated. Downregulation of MyoD instead of PAX7 leads to withdrawal from the cell cycle and myogenic differentiation, causing the cell to return to seemingly quiescent state. Chromosomal aberrations relating to PAX7 have been linked to rhabdomysarcoma 2 (RMS2).

Immunogen

KLH-conjugated linear peptide corresponding to mouse PAX7.

Application

Anti-PAX7 Antibody, clone 2F12H4 is a Rat monoclonal antibody for detection of PAX7 also known as paired box homeotic gene 7, paired box protein Pax-7, paired domain gene 7 & has been validated in WB, ICC.

Immunocytochemistry: A previous lot of this antibody was shown to detect Pax7 localized in euchromatin of human Ewing’s
sarcoma cells. The antibodies are detected with Alexa Fluor^™ 488-conjugated anti-rat IgG. Each sample is counterstained with hoechst (Harada, A., et al. (2009). Hybridoma, 26:6. Hyb.2009.0039).

Research Category
Stem Cell Research

Research Sub Category
Transcription Factors

Quality

Evaluated by Western Blot in mouse fetal brain tissue lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected PAX7 in 10 µg of mouse fetal brain tissue lysate.

Target description

~ 55 kDa observed.
The calculated MW of PAX7 is 55 kDa; however, expression of PAX7 can have multiple isoforms. (Robert B., et al. (2008). Physiol. Genomics 33:41-49.)

Physical form

Format: Purified

Protein G Purified

Purified rat monoclonal IgG2bκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Mouse fetal brain tissue lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

ALEXA FLUOR is a trademark of Life Technologies

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable