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GF029

Sigma-Aldrich

Brain Derived Neurotrophic Factor, recombinant

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About This Item

UNSPSC Code:
12352200
eCl@ss:
32160405
NACRES:
NA.28

assay

>98% (SDS-PAGE and HPLC)
<0.1 ng/μg (Endotoxin level)

Quality Level

specific activity

(Determined by its ability to bind recombinant human TrkB Fc Chimera in a functional ELISA.)

UniProt accession no.

Gene Information

human ... BDNF(627)

General description

FUNCTION: SwissProt: P23560 # Promotes the survival of neuronal populations that are all located either in the central nervous system or directly connected to it. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability.
SIZE: 247 amino acids; 27818 Da
SUBUNIT: Monomers and homodimers. Binds to NTRK2/TRKB.
SUBCELLULAR LOCATION: Secreted.
PTM: The propeptide is N-glycosylated and glycosulfated. & Converted into mature BDNF by plasmin (PLG) (By similarity).
DISEASE: SwissProt: P23560 # Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS) [MIM:209880]; also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases.
SIMILARITY: SwissProt: P23560 ## Belongs to the NGF-beta family.

Application

Concentration range for most in vitro applications is 0.5 to 100 ng/mL. Responding cells are (partial list): Nerve, glial cells.

Physical form

Sterile filtered, lyophilized with no additives.

Storage and Stability

The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstitute in water to a concentration of 0.1-1.0 mg/mL. This solution can be diluted into water or other buffered solutions. Reconstituted BDNF should be stored in undiluted aliquots at -20°C for up to six months. Avoid repeated freeze/thaw cycles.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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