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Key Documents

ABC91

Sigma-Aldrich

Anti-HUNK Antibody

from rabbit, purified by affinity chromatography

Synonym(s):

Hormonally up-regulated neu tumor-associated kinase, B19, Serine/threonine-protein kinase MAK-V

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About This Item

UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41

biological source

rabbit

Quality Level

100

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

purified by

affinity chromatography

species reactivity

human

technique(s)

immunohistochemistry: suitable (paraffin)
western blot: suitable

NCBI accession no.

NP_055401

UniProt accession no.

P57058

shipped in

wet ice

target post-translational modification

unmodified

Gene Information

human ... HUNK(30811)

General description

Hormonally up-regulated neu tumor-associated kinase (HUNK) belongs to the protein kinase superfamily and is an SNF1-related serine/threonine kinase that has a unique COOH-terminal (C-terminal) part without homology to any known protein. This protein is highly expressed in the mammary gland, kidney, and central nervous system.

Specificity

Other homologies: Mouse (87% sequence homology) and Rat (85% sequence homology).

Immunogen

GST-tagged recombinant protein corresponding to human HUNK.

Application

Immunohistochemistry Analysis: A 1:50-1:250 dilution from a representative lot detected HUNK in human brain and human cerebellum tissues.
This Anti-HUNK Antibody is validated for use in WB, IH(P) for the detection of HUNK.

Quality

Evaluated by Western Blot in SK-BR3 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected HUNK on 10 µg of SK-BR3 cell lysate.

Target description

~70 kDa observed. The calculated molecular weight is 80 kDa, however HUNK has been shown as a ~70 kDa band in western blots. (Gardner, H.P., et al. (2000). Development. 127:4493-4509.)

Analysis Note

Control
SK-BR3 cell lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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