1.10235

SPS agar

GranuCult^® plus, suitable for microbiology, for Clostridium spp.

• Synonym(s): Perfringens selective agar, Perfringens selective agar acc. to ANGELOTTI, ANGELOTTI Perfringens selective agar, Sulfite Polymyxin Sulfadiazine Agar
• UNSPSC Code: 41106213
• NACRES: NA.74

Properties

• sterility: non-sterile
• Quality Level: 200
• form: granular
• packaging: pkg of 500 g
• manufacturer/tradename: GranuCult^® plus
• storage condition: dry media (Keep tightly closed)
• technique(s): microbiological culture: suitable
• color: light brown
• pH: 6.9-7.1 (35 °C, 40 g/L in H₂O, after autoclaving)
• solubility: 40 g/L
• bulk density: 580 kg/m³
• application(s): food and beverages; microbiology
• storage temp.: 15-25°C
• suitability: Clostridium perfringens; Clostridium spp.

Description

General description

Sulfite Polymyxin Sulfadiazine (SPS) Agar contains a broad spectrum of nutrients. Most clostridia (including C. perfringens) reduce sulfite to sulfide, which reacts with iron citrate and causes the colonies to turn black. Other sulfite-reducing microorganisms are suppressed by polymyxin and sulfadiazine (sulfapyrimidine). The low sulfite content allows the growth of even sulfite-sensitive clostridia, which exhibit an adequate blackening of the colonies.

Application

SPS agar is suitable for the isolation and enumeration of Clostridium perfringens and Clostridium botulinum in all types of foodstuffs.

Features and Benefits

• GranuCult^® offers superior granulated culture media
• Safe and sustainable due to reduced risks associated with fine dust and toxic substance inhalation, resulting in a safer work environment
• Excellent wettability, solubility, and free-flowing properties
• Convenient, with minimal component separation and clumping, even under warm or humid conditions
• High batch-to-batch reproducibility
• Prolonged shelf life of up to five years
• High number of test strains exceeding all regulatory demands
• Granulation technology allows many supplements to be included, with no need to add these separately

Analysis Note

Appearance (clearness): clear
Appearance (colour): yellowish-brown
pH-value (25 °C): 6.8 - 7.2
Growth (Clostridium perfringens ATCC 10543): good to very good
Growth (Clostridium perfringens ATCC 13124 (WDCM 00007)): good to very good
Growth (Clostridium sporogenes ATCC 11437): good to very good
Growth (Escherichia coli ATCC 25922 (WDCM 00013)): none to fair
Growth (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): none to poor
Blacking (Clostridium perfringens ATCC 10543): +
Blacking (Clostridium perfringens ATCC 13124 (WDCM 00007)): +
Blacking (Clostridium sporogenes ATCC 11437): +
Blacking (Escherichia coli ATCC 25922 (WDCM 00013)): -
Blacking (Pseudomonas aeruginosa ATCC 27853 (WDCM 00025)): -
Incubation: 48 hrs.; 35 °C; aerobic.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk_germany: WGK 3