D2935

Decarboxylase broth base, Moeller

suitable for microbiology, NutriSelect^® Plus

• UNSPSC Code: 41171606
• NACRES: NA.85

Properties

• sterility: non-sterile
• Quality Level: 200
• product line: BioChemika
• form: powder
• shelf life: limited shelf life, expiry date on the label
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbe id | specific enzyme detection: suitable; microbiological culture: suitable
• final pH: 6.0±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; microbiology
• suitability: nonselective and differential for Citrobacter spp.; nonselective and differential for Enterobacter spp.; nonselective and differential for Escherichia coli; nonselective and differential for Klebsiella spp.; nonselective and differential for Proteus spp.; nonselective and differential for Salmonella spp.; nonselective and differential for Serratia spp.; nonselective and differential for Shigella spp.; nonselective and differential for coliforms; nonselective and differential for enterobacteriaceae

Description

Application

Used to differentiate bacteria on the basis of their ability to decarboxylate amino acids.

Components

Ingredients (g/L)
Peptic digest of animal tissue, 5.00
Beef extract, 5.00
Dextrose, 0.50
Bromocresol purple, 0.01
Cresol red, 0.005
Pyridoxal, 0.005

Preparation Note

Suspend 10.52 g of Decarboxylase Broth Base in 1000 ml of distilled water. Add 10 g of either L-Arginine (Cat. No. 11010), or L-Lysine (Cat. No. 62840), or L-Ornithine (Cat. No. 75470). Heat to dissolve the medium completely. When L-ornithine is added, readjust the pH. Dispense in 5 ml quantities in screwcapped tubes. Sterilize by autoclaving at 15 lbs. pressure (121°C) for 10 minutes.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves