49940
ONPG Disks
suitable for microbiology
Synonym(s):
β-Galactosidase Test Disks, 2-Nitrophenyl-β-D-galactopyranoside Disks
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About This Item
UNSPSC Code:
41171621
NACRES:
NA.85
Recommended Products
Agency
according to GB 4789.4-2016
product line
BioChemika
shelf life
limited shelf life, expiry date on the label
technique(s)
microbe id | specific enzyme detection: suitable
application(s)
environmental
food and beverages
microbiology
shipped in
wet ice
storage temp.
2-8°C
suitability
Escherichia coli
bacteria
General description
ONPG Disks are used to detect the presence of β-galactosidase, an enzyme found in lactose-fermenting organisms. Lactose utilization depends upon two enzymes: β-galactoside permease, which catalyzes transport of lactose into the cell, and β-galactosidase, which breaks down lactose into galactose and glucose. β-Galactosidase is not lactose specific and can act on simple galactosides including the ONPG (o-nitrophenyl-β-D-galactopyranose) substrate. ONPG hydrolysis results in the release of galactose, and the yellow chromogenic compound, o-nitrophenol. The test substrate, ONPG, does not depend on an induced or constitutive permease enzyme to enter the cell, therefore reactions are rapid and occur within a 24-hour period even for late lactose fermenters.
Packaging
Sterile filter paper discs (50 pcs, diameter 6 mm) impregnated with o-nitrophenyl-β-D-galactopyranose
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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