USP HPLC Analysis of L-Methionine on Ascentis® Express OH5
Conditions | |
|---|---|
| column | Ascentis Express OH5 15 cm x 4.6 mm, 5 µm particles (50347-U) |
| column temp. | 40 °C |
| mobile phase | [A] 0.005M Ammounium formate in Water (pH: 3.15 with Trifluoroacetic Acid) ), [B] Acetonitrile (30:70, A:B) |
| flow rate | 1.0 mL/min |
| pressure | 914 psi (63 bar) |
| sample | 0.8 mg/mL water:acetonitrile (1:1) |
| injection | 3 µL |
| detector | Agilent 380-ELSD |
Description | |
|---|---|
| Analysis Note | This USP application was generated by RTC on an Ascentis® Express OH5 column. |
| Categories | Analytical Chromatography, Amino Acids |
| Featured Industry | Life Science and Biopharma |
| suitability | application for HPLC |
Materials
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