Artificial reductant enhancement of the Lowry method for protein determination.

Artificial reductant enhancement of the Lowry method for protein determination.

Analytical biochemistry (1986-06-01)

E Larson, B Howlett, A Jagendorf

ABSTRAKT

Addition of dithiothreitol in the Lowry procedure 3 min after adding the Folin-Ciocalteau reagent produces immediate color development, with 35 to 60% greater absorbance per mass of protein used.

MATERIAŁY

Numer produktu

Marka

Opis produktu

F9252

Sigma-Aldrich

Folin & Ciocalteu′s phenol reagent, suitable for determination of total protein by Lowry method, 1.9-2.1 N