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808541

TSKgel® G3000SWXL (5 µm) HPLC Columns

L x I.D. 30 cm × 7.8 mm, diol phase

Synonim(y):

TSKgel® SWXL Columns

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Do Państwa/SKUDostępnośćCena netto
1 ea
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13 020,00 zł

Informacje o tej pozycji

UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
NB.21
L × i.d.:
30 cm × 7.8 mm
Particle size:
5 μm
Matrix active group:
diol phase
Pore size:
250 Å
Matrix:
silica particle platform

13 020,00 zł


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Nazwa produktu

TSKgel® G3000SWXL HPLC Column, phase diol, L × I.D. 30 cm × 7.8 mm, 5 μm particle size

material

stainless steel

agency

suitable for USP L20, suitable for USP L59

product line

TSKgel®

mol wt

1000 - 500,000

manufacturer/tradename

Tosoh 08541

parameter

≤0.5 M salt concentration, 0-100% aqueous soluble organic solvents, 1.2 mL/min flow rate, 10-30 °C temperature, 72 bar max. pressure

technique(s)

HPLC: suitable

L × I.D.

30 cm × 7.8 mm

matrix

silica particle platform

matrix active group

diol phase

particle size

5 μm

pore size

250 Å

operating pH

2.5-7.5

separation technique

size exclusion (SEC)

General description

Widely considered the premium columns for analyzing biopolymers by GFC, TSKgel SWXL columns feature high pore volume per unit column volume, low sample adsorption and excellent column efficiency, all contributing to unsurpassed sample resolution. With 5μm particles and 250Å pores, TSKgel G3000SWXL is an excellent choice for protein and large peptide separations. The fractionation range is applicable for globular protein samples up to 0.5 million Da.

Application

The TSKgel G3000SWXL columns are used: To separate monoclonal antibodies based on size exclusion.
  • For size-exclusion chromatography (SEC) to analyse PEGylated proteins, such as lysozyme. The column allows separation of different PEGylated isoforms based on their size.
  • To effectively separate and quantify high molecular weight species, oligomers, and monomers of IgG1 and IgG2 antibodies.
  • The columns provided clear resolution and accurate quantification essential for analyzing antibody stability and aggregation behavior.

Physical form

Shipped in 0.05% NaN3 and 0.1 M Na2SO4 in 0.1 M phosphate buffer, pH 6.7.

Other Notes

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Legal Information

TSKgel is a registered trademark of Tosoh Corporation
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Ta pozycja
808540805147806728
description

phase diol, L × I.D. 30 cm × 7.8 mm, 5 μm particle size

description

phase diol, L × I.D. 30 cm × 7.8 mm, 5 μm particle size

description

phase diol, L × I.D. 60 cm × 21.5 mm, 13 μm particle size

description

phase diol, L × I.D. 30 cm × 21.5 mm, 13 μm particle size

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

separation technique

size exclusion (SEC)

particle size

5 μm

particle size

5 μm

particle size

13 μm

particle size

13 μm

L × I.D.

30 cm × 7.8 mm

L × I.D.

30 cm × 7.8 mm

L × I.D.

60 cm × 21.5 mm

L × I.D.

30 cm × 21.5 mm

matrix active group

diol phase

matrix active group

diol phase

matrix active group

diol phase

matrix active group

diol phase

matrix

silica particle platform

matrix

silica particle platform

matrix

silica particle platform

matrix

silica particle platform

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable

technique(s)

HPLC: suitable




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Questions

1–5 of 5 Questions  
  1. I'm planning to purify peptides in the 1,000 to 5,000 Da range. Would this product be suitable for that application?

    1 answer
    1. Yes, this product is suitable for purifying peptides in the 1,000 to 5,000 Da range. The calibration range for this product is 1 KDa to 150 KDa.

      Helpful?

  2. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  3. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  4. Can this column be used on an Aekta system (FPLC)? Or is an HPLC required?

    1 answer
    1. This product is intended for use in HPLC. However, the item may be functional under FPLC conditions with the appropriate ÄKTA adapters.

      Helpful?

  5. Dears, Please advise whether or not Pre-column is required to be used with this item (Tosoh G3000 SWXL TSK Gel 7.8 mm x 30 cm, 5 µm)

    1 answer
    1. This product does not require a pre-column, it is used as an analytical column.

      Helpful?

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