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58354

Supelco

SUPELCOSIL LC-8-DB (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

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About This Item

Kod UNSPSC:
41115700
eCl@ss:
32110501
Informacje o cenach i dostępności nie są obecnie dostępne.

Nazwa produktu

SUPELCOSIL LC-8-DB HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm

agency

suitable for USP L7

Właściwości

endcapped

producent / nazwa handlowa

SUPELCOSIL

zakres etykietowania

6.0% carbon loading

Parametry

0-70 °C temperature
400 bar pressure (5801 psi)

metody

HPLC: suitable

dł. × śr. wewn.

25 cm × 4.6 mm

powierzchnia

170 m2/g

pokrycie powierzchni

surface coverage 3.2 μmol/m2

Matryca

silica gel, spherical particle platform

grupa aktywna macierzy

C8 (octyl) phase

wielkość cząstki

5 μm

wielkość porów

120 Å

Zastosowanie

food and beverages

metoda separacji

reversed phase

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Opis ogólny

SUPELCOSIL LC-DB phases are specially deactivated for basic compounds. These columns provide less retention, better peak shape, and higher efficiency for organic bases than can be obtained on conventional reversed-phase columns.

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Informacje prawne

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
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Jadwiga Piwowarska et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 805(1), 1-5 (2004-04-29)
A high-performance liquid chromatographic method is described for determination of lidocaine (2-(dietyloamino)-N-(2,6-dimetylofenylo) acetamid) and its metabolite, monoethylglycine xylidide (MEGX), in human serum containing various concentration of bilirubin. Lidocaine and its metabolite were extracted from human serum using dichloromethane. After separation
K Ary et al.
Journal of pharmaceutical and biomedical analysis, 26(2), 179-187 (2001-07-27)
A reversed-phase high-performance liquid chromatographic method with coulometric and UV detection has been developed for the simultaneous determination of morphine, morphine-3-glucuronide and morphine-6-glucuronide. The separation was carried out by using a Supelcosil LC-8 DB reversed-phase column and 0.1 M potassium
P K Kunicki
Journal of chromatography. B, Biomedical sciences and applications, 750(1), 41-49 (2001-02-24)
A HPLC-UV determination of clobazam and N-desmethylclobazam in human serum and urine is presented. After simple liquid-liquid extraction with dichloromethane the compounds and an internal standard diazepam were separated on a Supelcosil LC-8-DB column at ambient temperature under isocratic conditions
L Dal Bo et al.
Journal of chromatography. B, Biomedical applications, 665(2), 404-409 (1995-03-24)
A simple HPLC method has been developed for the determination of ticlopidine in human plasma. Plasma samples were buffered at pH 9 and extracted with n-heptane-isoamyl alcohol (98.5:1.5, v/v). Imipramine was used as internal standard. Chromatography was performed isocratically with
M Molíková et al.
Journal of chromatography. A, 1217(8), 1305-1312 (2010-01-12)
The availability of ionic liquids (ILs) in wide areas of application often results in the requirement on their determination. The attention is also often focused on the knowledge of hydrophobicity as it plays a key role in the biological effects

Questions

1–4 of 4 Questions  
  1. Do you have a cleaning procedure for Supelcosil LC-8DB HPLC column, Product 58354?

    1 answer
    1. Yes.

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  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  3. What does DB stand for in Supelcosil LC-8DB HPLC column, Product 58354?

    1 answer
    1. DB stands for "deactivated base", and refers to the base treatment of the packing in this column. Without this treatment, basic compounds will adsorb to active acidic silanol sites.  If starting a new method, Supelco suggests using a new-technology/pure-silica C8, such as the Ascentis C8.

      Helpful?

  4. Can the Supelcosil LC-8DB HPLC column, Product 58354, be used for acidic compounds?

    1 answer
    1. No, it is base treated, therefore acidic compounds will be adsorbed by the base treatment, resulting is peak tailing.

      Helpful?

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