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Y2377

2x YT medium

Powder microbial growth medium

Synonim(y):

2x Yeast Extract Tryptone medium, 2xTY medium, 2xYT Medium, 2xYT media

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Rozmiar/SKUDostępnośćCena netto
250 g
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278,00 zł

Informacje o tej pozycji

NACRES:
NA.85
UNSPSC Code:
41106225

278,00 zł


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grade

Molecular Biology

Quality Segment

sterility

non-sterile

form

powder

technique(s)

microbiological culture: suitable

pH

6.8-7.2(3.1% solution)

application(s)

food and beverages
microbiology

storage temp.

room temp

suitability

nonselective for Escherichia coli, nonselective for coliforms

General description

2xYT (2x Yeast Extract Tryptone medium) is a standard microbial growth medium used for the cultivation of E. coli and M13 bacteriophage. This nutrient-rich microbial broth contains peptides, amino acids, and water-soluble vitamins in a low-salt formulation.

Application

2x YT medium has been used for culturing E. coli BL21 for cloning , E. coli SS320 cells for scaffold synthesis , and NEB 5-alpha competent E. coli cells for protospacer adjacent motif (PAM) binding assays. It has also been used in the culturing of Agrobacterium tumefaciens and E. coli DH10B.

Features and Benefits

2xYT powder provides:
  • Standard formulation
  • An inexpensive alternative to liquid

Preparation Note

1. Stir to suspend 31 g of 2xYT Microbial Medium Powder in 1 L of water.
2. Heat to boiling while stirring to dissolve all ingredients completely.
3. Autoclave for 15 minutes at 121 °C.
Add 31 g powder in 1L water. Heat to boiling while stirring to dissolve powder. Autoclave for 15 minutes at 121°C to sterilize. Allow to cool before making additions, such as antibiotics (if desired).

Other Notes

16 g/L Tryptone
10 g/L Yeast Extract
5 g/L NaCl
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Ta pozycja
Y1003T9179N3643
description

Powder microbial growth medium

description

Liquid microbial growth medium

description

EZMix powder microbial growth medium

description

Powder microbial growth medium

form

powder

form

liquid

form

powder

form

powder

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

technique(s)

microbiological culture: suitable

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

suitability

nonselective for Escherichia coli, nonselective for coliforms

sterility

non-sterile

sterility

sterile; 0.2 μm filtered

sterility

non-sterile

sterility

non-sterile

application(s)

food and beverages
microbiology

application(s)

microbiology

application(s)

agriculture
food and beverages
microbiology

application(s)

food and beverages
microbiology

pH

6.8-7.2(3.1% solution)

pH

6.5-7.5

pH

-

pH

6.8-7.2(2.2% solution)


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Klasa składowania

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)



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Questions

1–2 of 2 Questions  
  1. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  2. What is the difference among the 2x YT Media products?

    1 answer
    1. 2x YT Media is available in liquid (Y1003), powder (Y2377) and EZMix™ powder formats (Y2627).

      Helpful?

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