V4014

Anti-V5-Cy3^™ antibody, Mouse monoclonal

purified immunoglobulin, ~1 mg/mL, clone V5-10

• Synonym(s): Monoclonal Anti-V5
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD03457579
• Conjugate: CY3 conjugate
• Clone: V5-10, monoclonal
• Application: ICC
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: CY3 conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: V5-10, monoclonal
• concentration: ~1 mg/mL
• technique(s): immunocytochemistry: 2.0-4.0 μg/mL
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

Application

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag.[1][2] This tag facilitates the detection, isolation and purification of the proteins.[3] Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

Legal Information

Cy3 is a trademark of Cytiva

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable