Unfortunately, this product has not been sequenced. In general, Ubiquitin is fairly conserved across species. Please see the link below to review a publication that reports that human and bovine ubiquitin are identical:
https://www.nature.com/articles/255423a0
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U6253
Ubiquitin from bovine erythrocytes
BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder
Synonim(y):
ATP-dependent proteolytic factor, Ub
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
5 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 231,00 zł |
25 mg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 796,00 zł |
Informacje o tej pozycji
Form:
essentially salt-free, lyophilized powder
Assay:
≥98% (SDS-PAGE)
Biological source:
bovine erythrocytes
231,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócbiological source
bovine erythrocytes
Quality Segment
product line
BioUltra
assay
≥98% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
storage condition
(Tightly closed. Dry)
technique(s)
western blot: suitable
impurities
salt, essentially free
solubility
water: 1 mg/mL, clear, colorless
UniProt accession no.
storage temp.
2-8°C
Gene Information
bovine ... LOC(101902760)
General description
Research area: Cancer
Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.
Ubiquitin is a highly conserved regulatory protein. It is found in all eukaryotic cells and is virtually identical across all forms of life including yeast, humans, and plants. ubiquitin structure contains seven Lys residues and an N-terminus, all of which are target sites for ubiquitination.
This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.
Application
Ubiquitin from bovine erythrocytes has been used to study the role of exogenous ubiquitin in chronic β-adrenergic receptor (β-AR)-stimulated myocardial remodeling. It has also been used to test the inhibitor of nuclear factor kappa-B kinase subunit beta (IKKβ) ubiquitylation.
Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.
Ubiquitin from bovine erythrocytes can be used for in vitro ubiquitinylation assay. The product can also be used as a marker in western blotting.
Biochem/physiol Actions
Ubiquitination is a post-translational modification process where ubiquitin-protein is attached to a substrate protein. Ubiquitination plays a vital role in the regulation of cellular signaling in various biological processes such as apoptosis, protein processing, immune response, and DNA repair. Ubiquitination mediates protein degradation via the ubiquitin-proteasome pathway. Ubiquitination is implicated in various cellular signaling pathways. Polyubiquitination modulates the signal activation of NF-κ-B inhibitor alpha (IkB-α) in the inflammatory signaling pathway. Elevated levels of ubiquitin have been observed in various diseases such as parasitic and allergic diseases, alcoholic liver disease, type 2 diabetes, β2-microglobulin amyloidosis, and chronic hemodialysis.
Preparation Note
Ubiquitin from bovine erythrocytes can dissolved in water at 1 mg/ml to yield a clear, colorless solution.
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Ta pozycja | |||
|---|---|---|---|
| description BioUltra, ≥98% (SDS-PAGE), essentially salt-free, lyophilized powder | description ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), lyophilized powder | description essentially salt-free, lyophilized powder, ≥10,000 BAEE units/mg protein (E1%/280, after activation to trypsin) | description BioUltra, ≥98% (SDS-PAGE), lyophilized powder, essentially salt free |
| technique(s) western blot: suitable | technique(s) mass spectrometry (MS): suitable | technique(s) mass spectrometry (MS): suitable | technique(s) ligand binding assay: suitable |
| biological source bovine erythrocytes | biological source human | biological source bovine pancreas | biological source bovine testis |
| assay ≥98% (SDS-PAGE) | assay ≥95% (SDS-PAGE) | assay 85-100% (UV) | assay ≥98% (SDS-PAGE) |
| form essentially salt-free, lyophilized powder | form lyophilized powder | form essentially salt-free, lyophilized powder | form lyophilized powder |
| storage condition (Tightly closed. Dry) | storage condition - | storage condition - | storage condition (Keep container tightly closed in a dry and well-ventilated place) |
| solubility water: 1 mg/mL, clear, colorless | solubility 0.05 M Tris pH 7.5: ≥10 mg/mL, 0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless | solubility H2O: soluble 10 mg/mL | solubility - |
Klasa składowania
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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The uniprot id provided on this website is for human poly Ubiquitin. Can you tell me the exact sequence of monoubiquitin that you have purified from bovine erythrocytes?
1 answer-
Helpful?
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Could you provide information about potential chemical residue in the product? Specifically, I would like to know if ethanol and/or acetone were used during the isolation and production process. I have detected some candidate adducts from this product and need to exclude the possibility that they originated from an artificial source.
1 answer-
The available testing information is limited to what is provided on the Certificate of Analysis (CofA). The specific method of production is considered proprietary; however, some general information about the production process can be shared. The details are as follows:
The final product should not contain any additives or stabilizers. The process involves overnight dialysis with deionized water to remove any remaining salts or additives. Subsequently, the product is removed from dialysis, filtered, and lyophilized. It is improbable that any ethanol or acetone would remain after the lyophilization process.
Helpful?
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I would like to know how this Ubiquitin is expressed and purified.
1 answer-
This product is from bovine red blood cells. It is not a recombinant product. The production process is considered proprietary, however it includes heat treatment as well as purification by chromatography and dialysis.
Helpful?
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