U4758

Anti-U2AF⁶⁵ antibody, Mouse monoclonal

clone MC3, purified from hybridoma cell culture

• Synonim(y): Anti-U2AF65
• Numer MDL: MFCD03456068
• Kod UNSPSC: 12352203
• NACRES: NA.41

Właściwości

• pochodzenie biologiczne: mouse
• Poziom jakości: 200
• białko sprzężone: unconjugated
• forma przeciwciała: purified immunoglobulin
• rodzaj przeciwciała: primary antibodies
• klon: MC3, monoclonal
• Postać: buffered aqueous solution
• masa cząsteczkowa: antigen ~65 kDa
• reaktywność gatunkowa: human, rat, Xenopus, mouse
• opakowanie: antibody small pack of 25 μL
• metody: electron microscopy: suitable; immunocytochemistry: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; indirect ELISA: suitable; microarray: suitable; western blot: 0.1-0.2 μg/mL using total cell extract from HeLa cells
• izotyp: IgG2b
• numer dostępu UniProt: P26368
• Warunki transportu: dry ice
• temp. przechowywania: −20°C
• docelowa modyfikacja potranslacyjna: unmodified
• informacje o genach: human ... U2AF2(11338) ; mouse ... U2af2(22185) ; rat ... U2af2(308335)

Opis

Opis ogólny

Monoclonal Anti-U2AF⁶⁵ (mouse IgG2b isotype) is derived from the MC3 hybridoma produced by the fusion of mouse myeloma cells (Ag8.653 cells) and splenocytes from BALB/c mice immunized with recombinant human U2AF⁶⁵. U1, U2, U4/U6, and U5 are small nuclear riboprotein (snRNPs) that are major subunits of spliceosomes. Spliceosomes also contain non snRNPs proteins such as alternative splicing factor/splicing factor 2 (ASF/SF2), 35 kDa spliceosomal component (SC-35) and U2 snRNP auxiliary splicing factor (U2AF). U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵) is a splicing factor which has three C-terminal RNA recognition motifs (RRMs) and an N-terminal arginine/serine-rich (RS) domain. This gene is mapped to human chromosome 19q13.42. Splicing factor U2AF⁶⁵kDa subunit is a non-snRNP protein component of spliceosome complexes.

Immunogen

recombinant human U2AF⁶⁵.

Zastosowanie

Monoclonal Anti-U2AF⁶⁵ antibody produced in mouse has been used in:

• RNA immunoprecipitation
• immunoblotting
• chromatin immunoprecipitation (ChIP)
• enzyme-linked immunosorbent assay (ELISA)
• immunohistochemistry
• immunocytochemistry
• immunoelectron microscopy

Działania biochem./fizjol.

U2 snRNP auxiliary factor 65 kDa (U2AF⁶⁵)/U2AF2 helps to supply the small nuclear ribonucleoprotein (snRNP) U2, which is subunit of the spliceosome. It can induce the exclusion of alternative exons. U2AF⁶⁵ primarily regulates the splicing of pre-mRNA. The serine-rich (RS) repeats at the N-terminal region act as a signalling factor for nuclear localization. (U2AF⁶⁵) and (U2AF³⁵) are components of the splicing factor U2AF that transports between the cytoplasm and the nucleus.

Postać fizyczna

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

Oświadczenie o zrzeczeniu się odpowiedzialności

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Informacja o środkach bezpieczeństwa

• Kod klasy składowania: 10 - Combustible liquids
• Klasa zagrożenia wodnego (WGK): WGK 2
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable