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T8778

Trehalase from porcine kidney

buffered aqueous glycerol solution, ≥1.0 units/mg protein

Synonim(y):

α,α-Trehalose glucohydrolase

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Do Państwa/SKUDostępnośćCena netto
1 unit
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681,00 zł
5 units
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2330,00 zł

Informacje o tej pozycji

Numer CAS:
9025-52-9
Numer WE:
3.2.1.28 (BRENDA, IUBMB)
UNSPSC Code:
12352204
NACRES:
NA.54
MDL number:
MFCD00132462
Specific activity:
≥1.0 units/mg protein
Concentration:
0.5-10.0 mg/mL protein (BCA)

681,00 zł

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form

buffered aqueous glycerol solution

Quality Segment

200

specific activity

≥1.0 units/mg protein

concentration

0.5-10.0 mg/mL protein (BCA)

storage temp.

−20°C

General description

Trehalase is a glycosidase that occurs in many forms and is found in all tissues. It belongs to the glycoside hydrolase 37 (GH37) family of the carbohydrate-active enzyme (CAZy) classification.
Trehalose is the main haemolymph sugar in most insects and is potentially a prime target for an invading pathogenic fungus.

Application

Trehalase has been used:
  • in a study to assess changes in carbohydrate metabolism in Plasmopara viticola-infected grapevine leaves
  • in a study to investigate growth arrest by trehalose-6-phosphate
  • to quantify the intracellular trehalose in Lactobacillus casei cells

Biochem/physiol Actions

Trehalase catalyzes the hydrolysis of trehalose to produce two glucose units. It mediates flight metabolism, molting induced chitin production and tolerance towards cold temperature.

Physical form

Solution in 50% glycerol containing 1% Triton™ X-100 and 25 mM potassium phosphate, pH 6.5

Other Notes

One unit will convert 1.0 μmole of trehalose to 2.0 μmoles of glucose per min at pH 5.7 at 37 °C (liberated glucose determined at pH 7.5).

Legal Information

Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
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Ta pozycja
T0167625625890808P
Trehalase from porcine kidney buffered aqueous glycerol solution, ≥1.0 units/mg protein

Sigma-Aldrich

T8778

Trehalase from porcine kidney

D-(+)-Trehalose dihydrate from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

Sigma-Aldrich

T0167

D-(+)-Trehalose dihydrate

Trehalose, Dihydrate Non-reducing disaccharide which yields 2 moles of D-glucose upon acid hydrolysis. Inhibits polyglutamine-mediated protein aggregation both in vivo and in vitro.

Millipore

625625

Trehalose, Dihydrate

22:0 Trehalose Avanti Research™ - A Croda Brand

Avanti

890808P

22:0 Trehalose

description

buffered aqueous glycerol solution, ≥1.0 units/mg protein

description

from Saccharomyces cerevisiae, powder, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99%

description

Non-reducing disaccharide which yields 2 moles of D-glucose upon acid hydrolysis. Inhibits polyglutamine-mediated protein aggregation both in vivo and in vitro.

description

Avanti Research™ - A Croda Brand

specific activity

≥1.0 units/mg protein

specific activity

-

specific activity

-

specific activity

-

concentration

0.5-10.0 mg/mL protein (BCA)

concentration

-

concentration

-

concentration

-

form

buffered aqueous glycerol solution

form

powder

form

solid

form

powder

storage temp.

−20°C

storage temp.

-

storage temp.

15-25°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

-

Klasa składowania

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–4 of 4 Questions  

  1. How much volume of trehalase enzyme is provided in a vial o1 1 Unit enzyme. Could you share the technical bulletin of trehalase.

    1 answer

    1. The volume of this product is lot specific and may be calculated using data provided in the Certificate of Analysis. For example, a reported protein concentration of 0.8 mg protein/mL with an activity of 2.6 units/mg protein would have a volume of 384 uL for the 1 unit pack size and 1.92 mL for the 5 unit pack size. A datasheet is not provided for this item. Please see the links below to review other helpful documentation.

      Sample or lot specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/t8778#product-documentation

      Enzymatic assay method:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/120/796/t8778enz.pdf

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  2. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

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  3. How is Product T8778, Trehalase from porcine kidney prepared?

    1 answer

    1. The actual method of preparation utilized by Sigma for Product T8778, Trehalase is confidential, but does include ion exchange and size exclusion chromatography. A method of preparation for Trehalase has been described in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987).

      Helpful?

  4. What is the molecular weight of Product T8778, Trehalase from porcine kidney?

    1 answer

    1. The molecular weight of Product T8778, Trehalase from porcine kidney has an estimated molecular weight of 80,000 daltons upon SDS-PAGE, as described by Yoshitaka Yoneyama in Archives of Biochemistry and Biophysics, 255, 168 - 175 (1987). This molecular weight is similar to that reported for mouse Trehalase which has an estimated molecular weight by gel filtration on Sephadex G-200 of 73,000 daltons, as described in Anais Da Academia Brasileira De Ciencias, 51, 151 - 158 (1979).

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