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SRP3098

IL-8 (77aa) (CXCL8) human

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

Synonim(y):

CXCL8, NAP-1, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF)

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Do Państwa/SKUDostępnośćCena netto
25 μg
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1320,00 zł

Informacje o tej pozycji

NACRES:
NA.32
UNSPSC Code:
12352202

1320,00 zł


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biological source

human

recombinant

expressed in E. coli

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

mol wt

8.9 kDa

packaging

pkg of 25 μg

technique(s)

cell culture | mammalian: suitable

impurities

<0.1 EU/μg endotoxin, tested

color

white to off-white

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... IL8(3576)

General description

Interleukin-8 (IL-8) is a proinflammatory CXC chemokine that can signal through the C-X-C motif chemokine receptors 1 and 2 (CXCR1 and 2). It is also called as C-X-C motif chemokine ligand 8 (CXCL8). IL-8 is secreted by monocytes and endothelial cells. The gene encoding IL-8 is localized on human chromosome 4q13.3. Recombinant human IL-8 (endothelial-derived) is an 8.9kDa protein containing 77 amino acid residues.

Application

IL-8 (77aa) (CXCL8) human has been used as a G protein-coupled receptor (GPCR) agonist.

Biochem/physiol Actions

Interleukin-8 (IL-8) chemoattracts and activates neutrophils. It is involved in guiding neutrophils and oligodendrocytes to sites of infection. The protein also stimulates angiogenesis and tumor growth. It is also associated with inflammatory diseases.

Physical form

Lyophilized with no additives.

Preparation Note

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a carrier protein (example 0.1% BSA) and store in working aliquots at -20°C to -80°C.

Other Notes

AVLPRSAKEL RCQCIKTYSK PFHPKFIKEL RVIESGPHCA NTEIIVKLSD GRELCLDPKE NWVQRVVEKF LKRAENS
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Ta pozycja
SRP3311SRP3083SRP3310
description

recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

description

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC)

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

recombinant

expressed in E. coli

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

technique(s)

-

biological source

human

biological source

human

biological source

human

biological source

human

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (SDS-PAGE), ≥98% (HPLC)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

assay

≥98% (HPLC), ≥98% (SDS-PAGE)

form

lyophilized

form

lyophilized

form

lyophilized

form

lyophilized

mol wt

8.9 kDa

mol wt

8.4 kDa

mol wt

17.3 kDa

mol wt

18.0 kDa


Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable



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Questions

  1. AFTER THE INITIAL RECONSTITUTION IN WATER, CAN THIS BE DILUTED IN PBS WITH 10% FBS?

    1 answer
    1. After reconstitution with water. For extended storage, it is recommended to further dilute the solution in a buffer containing a carrier protein (example 0.1% BSA) and store it in working aliquots at -20°C to -80°C. As BSA is a significant component of FBS this would meet the long-term storage recommendation criteria.

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