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SRP0288
ATP Citrate Lyase Active human
recombinant, expressed in baculovirus, ≥90% (SDS-PAGE)
Synonim(y):
ACLY, ATP-citrate synthase, ATPCL, CLATP
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| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
10 μg | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 3420,00 zł |
Informacje o tej pozycji
NACRES:
NA.54
UNSPSC Code:
12352204
Specific activity:
≥1.2 pmol/min-μg
Assay:
≥90% (SDS-PAGE)
Recombinant:
expressed in baculovirus
3420,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
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Pozwól nam pomócGeneral description
Human ATP citrate lyase (ACLY) (GenBank Accession No. NM_001096), full length, with N-terminal GST tag, MW = 147 kDa, expressed in baculovirus expression system.
Application
Active human ATP citrate lyase is useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling. Active human ATP citrate lyase has been used in a study to ascertain the nature of the catalytic phosphorylation that initiates the ACL reaction, and to identity the active site residues involved. Active human ATP citrate lyase has also been used in a study to analyze tumor metabolism to reveal mitochondrial glucose oxidation in genetically diverse human glioblastomas.
Biochem/physiol Actions
ATP citrate lyase is an enzyme involved in fatty acid synthesis that generates cytosolic acetyl-CoA and oxaloacetate from citrate and CoA. ATP citrate lyase is often upregulated in cancer.
Physical form
Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20 and 10% glycerol.
Preparation Note
Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.
Other Notes
One unit is defined as the amount of enzyme required to convert 1 pmol of ADP to ATP/min at 37°C.
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Ta pozycja | |||
|---|---|---|---|
| description recombinant, expressed in baculovirus, ≥90% (SDS-PAGE) | description affinity isolated antibody | description - | description clone 5F8D11, from mouse |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay - |
| Gene Information mouse ... ACLY(47) | Gene Information human ... ACLY(47) | Gene Information human ... ACLY(47) | Gene Information human ... ACLY(47) |
| specific activity ≥1.2 pmol/min-μg | specific activity - | specific activity - | specific activity - |
| form aqueous solution | form buffered aqueous solution | form buffered aqueous solution | form - |
| recombinant expressed in baculovirus | recombinant - | recombinant - | recombinant - |
| storage temp. −70°C | storage temp. −20°C | storage temp. −20°C | storage temp. - |
Klasa składowania
10 - Combustible liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
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