The bead size for the V5 antibody ranges from 20 to 75 µm.
The affinity of the V5 antibody used (clone V5-10) has not been characterized. However, there is a relevant publication that contains this information for clone V5-10, which may serve as a useful reference:
- Title: HiBiT-qIP, HiBiT-based quantitative immunoprecipitation, facilitates the determination of antibody affinity under immunoprecipitation conditions
- Journal: Sci Rep. 2019 May 3;9(1):6895
- DOI: 10.1038/s41598-019-43319-y
- PMID: 31053795
You can access the publication at https://pubmed.ncbi.nlm.nih.gov/31053795/.
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Informacje o tej pozycji
General description
Application
Features and Benefits
- Kulki są przygotowywane z oczyszczonym przez powinowactwo przeciwciałem anty-V5.
- Monoklonalne przeciwciało anty-V5 jest wytwarzane przez fuzję mysich komórek szpiczaka i splenocytów myszy BALB/c.
- Stosunek białka do kulki wynosi około 2 mg/ml.
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Ta pozycja | |||
|---|---|---|---|
| conjugate magnetic beads | conjugate magnetic beads | conjugate peroxidase conjugate | conjugate agarose conjugate |
| form suspension | form suspension | form lyophilized powder | form - |
| storage temp. 2-8°C | storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C |
| shipped in ambient | shipped in wet ice | shipped in - | shipped in wet ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Klasa składowania
10 - Combustible liquids
wgk
WGK 3
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Do you have information about the affinity of the antibodies used for the V5 tag? Additionally, what is the bead size to which the V5 antibody is conjugated?
Do you have information about the affinity of the antibodies used for the V5 tag? Additionally, what is the bead size to which the V5 antibody is conjugated?
1 answer-
Helpful?
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Anti-V5 Magnetic Beads (SAE0203-1ML) Can these beads be stripped and sanitized so that they can be reused for protein purification?
1 answer-
The binding capacity of these beads is 20-62 nmoles of V5-tagged fusion protein per 1 mL of settled resin. While the product may be reused, a protocol and the reuse capacity has not been determined. For reuse it is recommended to wash the resin thoroughly following elution. Please see the link below to review the product datasheet:
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/275/574/sae0203pis-ms.pdfHelpful?
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