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Merck

S4881

Staphylococcal enterotoxin B from Staphylococcus aureus

Synonim(y):

Enterotoxin B, Staphylococcal, SEB

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Wybierz wielkość

1 MG

1430,00 zł

5 MG

5760,00 zł

1430,00 zł


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Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352202
NACRES:
NA.25
MDL number:

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contains

sodium phosphate buffer as balance

composition

Protein, ~25% Lowry

availability

not available in USA

impurities

Staphylococcal enterotoxin A (SEA), essentially free

application(s)

metabolomics
vitamins, nutraceuticals, and natural products

storage temp.

2-8°C

Quality Level

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Ta pozycja
S9399324798T5662
application(s)

metabolomics
vitamins, nutraceuticals, and natural products

application(s)

-

application(s)

-

application(s)

-

Quality Level

300

Quality Level

200

Quality Level

100

Quality Level

200

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

impurities

Staphylococcal enterotoxin A (SEA), essentially free

impurities

Staphylococcal enterotoxin B (SEB), essentially free

impurities

-

impurities

-

contains

sodium phosphate buffer as balance

contains

sodium phosphate buffer as balance

contains

-

contains

-

composition

Protein, ~25% Lowry

composition

Protein, ~10% Lowry

composition

-

composition

Contains ~50% protein (Lowry); balance primarily sodium chloride and sodium phosphate buffer.

General description

Staphylococcal Enterotoxin B (SEB) is a potent exotoxin produced by Staphylococcus aureus, classified as a superantigen due to its unique ability to activate T-cells non-specifically. This activation occurs through the cross-linking of T-cell receptors (TCRs) and major histocompatibility complex (MHC) class II molecules, leading to a massive release of cytokines and an exaggerated immune response. SEB is associated with food poisoning and toxic shock syndrome, making it a critical agent for studying the mechanisms of bacterial pathogenesis and immune dysregulation.
In addition to its role in infectious disease research, SEB serves as a valuable tool for investigating autoimmune conditions and the development of immunotherapies. Its ability to induce T-cell activation and proliferation makes it an important reagent for exploring T-cell biology, including differentiation, memory formation, and the effects of various immunomodulators.

Application

Staphylococcal enterotoxin B (SEB) which binds to MHC class II molecules and specific Vβ regions of T cell receptors (TCR), resulting in the activation of both monocytes/macrophages and T lymphocytes may be used as a superantigen and immune system activator. SEB may be used as a reference material in the development of SEB detection systems.

Biochem/physiol Actions

A superantigen for T-lymphocytes. Activator of the immune system that binds to major histocompatibility complex (MHC) antigens and CD28.[1]

Packaging

Package size based on protein content.
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pictograms

Skull and crossbones

signalword

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 1 Oral - Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.

Odwiedź Bibliotekę dokumentów

A Herman et al.
Annual review of immunology, 9, 745-772 (1991-01-01)
Superantigens combine with MHC class-II molecules to form the ligands that stimulate T cells via the V beta element of the T-cell receptor. Two groups of superantigens have been described so far: first, endogenous murine products that include the Mls
Bacterial superantigens
Proft, T. and Fraser, J.D.
Clinical and Experimental Immunology, 133, 299-299 (2008)
Clarifying the mechanism of superantigen toxicity.
John D Fraser
PLoS biology, 9(9), e1001145-e1001145 (2011-09-21)
Oliver G Weingart et al.
Sensors (Basel, Switzerland), 12(2), 2324-2339 (2012-03-23)
Prevalent incidents support the notion that toxins, produced by bacteria, fungi, plants or animals are increasingly responsible for food poisoning or intoxication. Owing to their high toxicity some toxins are also regarded as potential biological warfare agents. Accordingly, control, detection
Winnie Bergstedt et al.
PloS one, 5(6), e11277-e11277 (2010-07-02)
Tuberculin is still the only available skin test reagent for the diagnosis of mycobacterial infection. The product has a remarkable sensitivity, but poor specificity. Previous studies, including two human phase I clinical trials, have indicated that rdESAT-6 has a potential

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Questions

1–5 of 5 Questions  
  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer
    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

      Helpful?

  2. Hi I would like to know how to store the Staphylococcal enterotoxin B after resuspend in H2O. Is it stable in aqueous? What's the recommend temperature to store them in aqueous?

    1 answer
    1. At refrigerated or room temperature, solutions prepared in 0.05 M phosphate buffer, pH 4–7.3 were found to be stable for at least 1 week. For long term storage, aliquot and freeze stock solutions at -20°C for up to 3 months. Please see the link below to review additional information available in the product datasheet:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/126/857/s4881pis.pdf

      Helpful?

  3. What is the difference in effects on T-lymphocyte proliferation between PHA and SEB? Thank you

    1 answer
    1. T-cells stimulated with SEB produce high levels of IFN-γ, while PHA-stimulated T-cells produce little or no IFN-γ. For more information, refer to the reference at the following link: https://pmc.ncbi.nlm.nih.gov/articles/PMC1906565/#:~:text=%2Dcells%20(Fig.-,1).,compared%20to%20CD4+%20T%2Dcells

      Helpful?

  4. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer
    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

      Helpful?

  5. こんにちは、この商品の容量をお聞きしたいのですが、1MGと表記されている場合、1miligramということでしょうか? 溶かしてから調剤したいのですが。 溶かして調剤する際のプロトコルはあるのでしょうか?

    1 answer
    1. Yes, the 1MG does stand for one milligram. The product is soluble in water at 4 mg/ml. It may also be dissolved in an aqueous buffer such as PBS. See the link below to the product information sheet for additional solution stability information:
      https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/126/857/s4881pis.pdf

      Helpful?

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