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P3910

Protease from Bacillus licheniformis

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

Synonim(y):

Proteinase from Bacillus licheniformis, Subtilisin A

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Do Państwa/SKUDostępnośćCena netto
500 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
1100,00 zł
5 x 500 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
4190,00 zł

Informacje o tej pozycji

Numer CAS:
UNSPSC Code:
12352204
eCl@ss:
32160410
EC Number:
232-752-2
NACRES:
NA.84
MDL number:

1100,00 zł


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form

lyophilized powder

Quality Segment

quality

for use in Total Dietary Fiber Assay, TDF-100A

mol wt

27 kDa

application(s)

food and beverages

shipped in

wet ice

storage temp.

2-8°C

General description

Subtilisin is a non-glycosylated single polypeptide chain without disulfide bonds and has a molecular weight of 27 KDa.

Application

This is a proteolytic enzyme isolated from the fermentation of Bacillus licheniformis. It is a serine endoproteinase with a broad specificity towards native and denatured proteins, and is active under alkaline conditions. It is for use in Total Dietary Fiber Assays (TDF-100A).

Biochem/physiol Actions

Subtilisin A is a member of the Serine S8 Endoproteinase family. It has broad specificity with a preference for a large uncharged residue in the P1 position. It hydrolyzes native and denatured proteins, and is active under alkaline conditions.
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate .

Other Notes

One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per minute at pH 7.5 at 37°C.
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Ta pozycja
P8038P5380P4860
description

lyophilized powder, for use in Total Dietary Fiber Assay, TDF-100A

description

Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder

description

Type VIII, lyophilized powder, 7-15 units/mg solid

description

≥2.4 U/g

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

aqueous solution

application(s)

food and beverages

application(s)

-

application(s)

-

application(s)

-

shipped in

wet ice

shipped in

wet ice

shipped in

-

shipped in

-

mol wt

27 kDa

mol wt

27 kDa

mol wt

27 kDa

mol wt

-

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

200


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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

target_organs

Respiratory system

Klasa składowania

11 - Combustible Solids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

dust mask type N95 (US), Eyeshields, Faceshields, Gloves



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Questions

1–3 of 3 Questions  
  1. Where can we find information of U/mg of this product?

    1 answer
    1. This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
      https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011

      Helpful?

  2. 本試薬の比活性 ユニット/mgをご教授ください。

    1 answer
    1. This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
      https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011

      Helpful?

  3. 본 제품에 대한 U/mg 값을 알고싶습니다.

    1 answer
    1. This product is intended for use as a component for the Total Dietary Fiber Assay kit, TDF-100A. Unfortunately, the activity is not determined. See the link below to review Protease from Bacillus licheniformis products that are tested for activity:
      https://www.sigmaaldrich.com/substance/proteasefrombacilluslicheniformis123459014011

      Helpful?

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