P1269

Phorbol 12,13-dibutyrate

≥98% (TLC), powder, PKC activator

• Synonim(y): PDBu
• Wzór empiryczny (zapis Hilla): C₂₈H₄₀O₈
• Numer CAS: 37558-16-0
• Masa cząsteczkowa: 504.61
• NACRES: NA.77
• PubChem Substance ID: 24898178
• UNSPSC Code: 12352211
• MDL number: MFCD00036783
• Beilstein/REAXYS Number: 6551234
• Assay: ≥98% (TLC)
• Form: powder

Właściwości

• Nazwa produktu: Phorbol 12,13-dibutyrate, ≥98% (TLC), powder
• Quality Segment: 100
• assay: ≥98% (TLC)
• form: powder
• color: clear light yellow
• solubility: H₂O: 30 μM, DMSO: soluble, acetone: soluble, ethanol: soluble
• storage temp.: −20°C
• SMILES string: CCCC(=O)O[C@@H]1[C@@H](C)[C@@]2(O)[C@@H](C=C(CO)C[C@@]3(O)[C@H]2C=C(C)C3=O)[C@@H]4C(C)(C)[C@]14OC(=O)CCC
• InChI: 1S/C28H40O8/c1-7-9-20(30)35-24-16(4)27(34)18(22-25(5,6)28(22,24)36-21(31)10-8-2)12-17(14-29)13-26(33)19(27)11-15(3)23(26)32/h11-12,16,18-19,22,24,29,33-34H,7-10,13-14H2,1-6H3/t16-,18+,19-,22-,24-,26-,27-,28-/m1/s1
• InChI key: BQJRUJTZSGYBEZ-YVQNUNKESA-N
• Gene Information: mouse ... Prkcd(18753)

Opis

Application

Phorbol 12,13-dibutyrate has been used as a protein kinase C (PKC) activator to study its effects on human organic anion transporter 4 (hOAT4) activity.[1] It has also been used as a PKC activator to analyze its effects on galectin-3 expression and matrix production in HL-1 cells.[2]

Biochem/physiol Actions

Phorbol 12,13-dibutyrate (PDBu) is a potent activator of protein kinase C (PKC). It is more hydrophilic than phorbol 12-myristate 13-acetate (PMA), which facilitates washing PDBu out of cells in tissue culture.[3] PDBu activates endothelial nitric oxide synthase expression in primary human umbilical vein endothelial cells, which in turn correlates with PKC α and ε expression.[4]

Features and Benefits

Less hydrophobic then PMA, making it easier to wash out of cells in tissue culture.

Preparation Note

Stock solution cannot be made in aqueous media; dissolve in a water-miscible organic solvent before dilution to working concentrations in aqueous media.

Disclaimer

Photosensitive

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Informacja o środkach bezpieczeństwa

• pictograms: GHS06,GHS08,GHS05
• signalword: Danger
• hcodes: H300 + H310 + H330,H314,H317,H334,H351
• pcodes: P202 - P260 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 1 Dermal - Acute Tox. 1 Inhalation - Acute Tox. 2 Oral - Carc. 2 - Eye Dam. 1 - Resp. Sens. 1 - Skin Corr. 1B - Skin Sens. 1
• Klasa składowania: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: dust mask type N95 (US), Eyeshields, Gloves