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P1261

Phosphorylase a from rabbit muscle

lyophilized powder, 20-30 units/mg protein

Synonim(y):

1,4-α-D-Glucan:orthophosphate α-D-glucosyltransferase

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Do Państwa/SKUDostępnośćCena netto
10 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
981,00 zł
25 mg
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
2010,00 zł

Informacje o tej pozycji

Numer CAS:
9032-10-4
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
232-878-8
MDL number:
MFCD00167428
Numer WE:
2.4.1.1 (BRENDA, IUBMB)
Specific activity:
20-30 units/mg protein
Biological source:
rabbit muscle

981,00 zł

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biological source

rabbit muscle

Quality Segment

200

form

lyophilized powder

specific activity

20-30 units/mg protein

purified by

2× crystallization

composition

Protein, 30-60% E1%/280

foreign activity

AMP-phosphatase, ATP-phosphatase, debrancher enzyme and phosphorylase phosphatase ≤0.1%, phosphoglucomutase ≤0.25%, phosphorylase b ≤30%

storage temp.

−20°C

General description

Phosphorylase A is the active form of glycogen phosphorylase which converts glycogen and orthophosphate (Pi) to glucose 1-phoshate (G-1-P).

Application

Phosphorylase a from rabbit muscle has been used to mix with glycogen for the preparation of limit dextrin. It has also been used to probe the array in oligosaccharide microarray analysis of phosphorylase activity.
Phosphorylase from rabbit muscle has been used in a study to assess the molecular mechanisms of oleanolic acid. [1] It has also been used in a study to describe the conversion of phosphorylase B to A, through a conversion enzyme in the presence of 32P-ATP. [2]

Biochem/physiol Actions

Phosphorylase participates in glycogenolysis. Deficiency of phosphorylase leads to several glycogen storage diseases with hepatomegaly.
Dimeric phosphorylase b is converted to the more active tetramer, phosphorylase a, by the action of phosphorylase kinase.
Phosphorylase A can be inhibited by these compounds: Polychlorinated biphenyls, polychlorinated biphenylols and polybrominated biphenyls. [3]

Physical form

Lyophilized powder containing β-glycerophosphate and EDTA

Other Notes

One unit will form 1.0 μmole of α-D-glucose 1-phosphate from glycogen and orthophosphate per min at pH 6.8 at 30 °C, measured in a system containing phosphoglucomutase, NADP, and glucose-6-phosphate dehydrogenase. (One μmolar unit is equivalent to ~45 Cori units.)
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Ta pozycja
P9544P6635G2267
Phosphorylase a from rabbit muscle lyophilized powder, 20-30 units/mg protein

Sigma-Aldrich

P1261

Phosphorylase a from rabbit muscle

Phosphoglucose Isomerase from rabbit muscle Type XI, lyophilized powder, ≥200 units/mg protein

Sigma-Aldrich

P9544

Phosphoglucose Isomerase from rabbit muscle

Phosphorylase b from rabbit muscle lyophilized powder, ≥20 units/mg protein, 2× crystallization

Sigma-Aldrich

P6635

Phosphorylase b from rabbit muscle

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle lyophilized powder, ≥75 units/mg protein

Sigma-Aldrich

G2267

Glyceraldehyde-3-phosphate Dehydrogenase from rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

biological source

rabbit muscle

specific activity

20-30 units/mg protein

specific activity

≥200 units/mg protein

specific activity

≥20 units/mg protein

specific activity

≥75 units/mg protein

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

purified by

2× crystallization

purified by

-

purified by

2× crystallization

purified by

-

Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–8 of 8 Questions  

  1. What is the buffer required for the enzyme activity?

    1 answer

    1. The final buffer that this product is in prior to lyophilization is 0.1 M B-glycerophosphate and 4 mM EDTA.
      A stock solution can be prepared in 40 mM glycerophosphate buffer, pH 6.8, containing 1 mM EDTA and 5 mM cysteine. EDTA is a chelating agent of copper and other metal ions, which causes oxidation of the protein. The presence of cysteine is to maintain the critical sulfhydryl group in the protein in the reduced state.
      When preparing a stock solution for assaying the activity, a 0.25 unit/ml solution of the protein in 40 mM glycerophosphate buffer, pH 6.8, at 30 °C containing 80 mM cysteine is used.

      Helpful?

  2. What is the solubility of Phosphorylase A, Product P1261?

    1 answer

    1. Sigma tests solubility of Phosphorylase A (P1261) in water at a concentration of 1 mg/ml.

      Helpful?

  3. What is the Department of Transportation shipping information for this product?

    1 answer

    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

  4. What is the molecular weight of Phosphorylase A, Product P1261?

    1 answer

    1. The molecular weight of Rabbit Phosphorylase A (tetramer) is 370,000 per Biochemistry 6 (1967) p. 3315 (which was determined by sedimentation and "experiments in a variety of buffers, temperatures, and ionic strength").  The molecular weight by sequence analysis (Proc. Natl. Acad. Sci. USA, 74, 4762-4766) is 97,412.

      Helpful?

  5. Has the amino acid sequence of phosphorylase from rabbit muscle been published?

    1 answer

    1. The sequence has been published. The amino acid composition is:Asp5l, Asn45, Thr35, Ser29, Glu64, Gln31, Pro36, Gly48, Ala63, Cys9, Val62, Met2l, Ile49, Leu79, Tyr36, Phe38, Trp12, Lys48, HiS22, and Arg63. Reference for the sequence and amino acid composition: K. Titani et al., Proc. Nat. Acad. Sci., USA, 74, 4762-4766 (1977) (see attached pdf).Product P1261 is phosphorylase a, which is the tetrameric form. Products P4649 and P6635 are phosphorylase b, the dimeric  form. The sequence information of the subunit would be the same for both forms.

      Helpful?

  6. How is Phosphorylase A, Product P1261, prepared?

    1 answer

    1. To prepare Phosphorylase A (P1261), Sigma uses a method similar to the one described in the J. Biol.Chem., 231, 65 (1958).

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  7. What is the final buffer Phosphorylase A, Product P1261, is in prior to lyophilization?

    1 answer

    1. The final buffer Phosphorylase A (P1261) is in prior to lyophilization is 0.1MB-glycerophosphate and 4 mM EDTA.

      Helpful?

  8. What is the pI of Phosphorylase A, Product P1261?

    1 answer

    1. The pI of Phosphorylase A (P1261) is 5.6 - 5.8.

      Helpful?

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