Przejdź do
MBD0040
Sonda Proteus FISH - Cy3
Probe for fluorescence in situ hybridization (FISH),20 μM in water
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
50 μL | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 4880,00 zł |
Informacje o tej pozycji
NACRES:
NA.55
UNSPSC Code:
12352200
4880,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócQuality Segment
technique(s)
FISH: suitable
fluorescence
λex 550 nm; λem 570 nm (Cy3)
shipped in
dry ice
storage temp.
−20°C
General description
Fluorescent In Situ Hybridization technique (FISH) is based on the hybridization of fluorescent labeled oligonucleotide probe to a specific complementary DNA or RNA sequence in whole and intact cells.1 Microbial FISH allows the visualization, identification and isolation of bacteria due to recognition of ribosomal RNA also in unculturable samples.2
FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms5 and aquatic systems 6) and plants 7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative (MBD0034/35) control probes, that accompany the specific probe of interest.
Proteus probe specifically recognizes Proteus species (see images of Proteus vulgaris and Proteus mirabilis). Moreover, it was shown that this probe recognizes Proteus penneri and Proteus hauseri.16
P. mirabilis and P. vulgaris are gram negative facultative anaerobic rod-shaped bacteria. Bacteria of the genus Proteus (Proteus spp.) of the family Enterobacteriaceae are opportunistic human pathogens, that often reside in the human intestine, and are responsible for wound and burn infections as well as skin, eye, ear, nose, throat, urinary tract, and gastrointestinal infections and bacteremias.8,9 Proteus genus that includes P. mirabilis, P. vulgaris, Proteus penneri and Proteus hauseri, are widespread in the environment and often serve as an indicator for soil or water fecal pollution.9
P. mirabilis causes up to 90% of all Proteus spp. infections and is more frequently associated with urinary tract infections then P. vulgaris.9 Proteus spp. have various virulence factors such as, fimbriae, flagella, enzymes (urease, proteases, and amino acid deaminases), and toxins (hemolysins and endotoxin).10
The most common infection involving P.mirabilis occurs when the bacteria, which is a member of the natural intestinal flora, moves to the urethra and urinary bladder causing urinary tract infection. The outer-membrane lipopolysaccharide (LPS) is considered an important virulence factor of Proteus.10 The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.11 A possible correlation between the abundance of
P.mirabilis in the intestine and obesity was suggested recently.12
P. vulgaris inhabits the intestinal tract of humans and animals and can be found in soil, water and feces.8 Moreover, it was also observed in fecal samples of healthy individuals.13
P. mirabilis has developed resistance to various classes of antibiotics, such as β-lactams aminoglycosides and tetracyclines.14 However, P. mirabilis strains are generally more susceptible to antimicrobials than are P. vulgaris,
P. penneri, and P. hauseri.15
FISH technique was successfully used for clinical detection of Proteus spp. in artificial urine medium and urine samples from patients with UTIs. The probe was able to detect 11 strains of P.mirabilis, 6 strains of P. vulgaris, 2 strains of P.penneri and one strain of P. hauseri.16 The probe can also be used to detect P. mirabilis and P. vulgaris pure culture (as described in the figure legends). FISH can also be implicated to detect Proteus spp. in colon sections embedded in paraffin.17,18 Moreover, FISH can be implicated to identify Proteus spp. in the gut of the medicinal leech.19
FISH technique can serve as a powerful tool in the microbiome research field by allowing the observation of native microbial populations in diverse microbiome environments, such as samples from human origin (blood3 and tissue4), microbial ecology (solid biofilms5 and aquatic systems 6) and plants 7. It is strongly recommended to include positive and negative controls in FISH assays to ensure specific binding of the probe of interest and appropriate protocol conditions. We offer positive (MBD0032/33) and negative (MBD0034/35) control probes, that accompany the specific probe of interest.
Proteus probe specifically recognizes Proteus species (see images of Proteus vulgaris and Proteus mirabilis). Moreover, it was shown that this probe recognizes Proteus penneri and Proteus hauseri.16
P. mirabilis and P. vulgaris are gram negative facultative anaerobic rod-shaped bacteria. Bacteria of the genus Proteus (Proteus spp.) of the family Enterobacteriaceae are opportunistic human pathogens, that often reside in the human intestine, and are responsible for wound and burn infections as well as skin, eye, ear, nose, throat, urinary tract, and gastrointestinal infections and bacteremias.8,9 Proteus genus that includes P. mirabilis, P. vulgaris, Proteus penneri and Proteus hauseri, are widespread in the environment and often serve as an indicator for soil or water fecal pollution.9
P. mirabilis causes up to 90% of all Proteus spp. infections and is more frequently associated with urinary tract infections then P. vulgaris.9 Proteus spp. have various virulence factors such as, fimbriae, flagella, enzymes (urease, proteases, and amino acid deaminases), and toxins (hemolysins and endotoxin).10
The most common infection involving P.mirabilis occurs when the bacteria, which is a member of the natural intestinal flora, moves to the urethra and urinary bladder causing urinary tract infection. The outer-membrane lipopolysaccharide (LPS) is considered an important virulence factor of Proteus.10 The immunological response against P. mirabilis LPS might play a role in rheumatoid arthritis.11 A possible correlation between the abundance of
P.mirabilis in the intestine and obesity was suggested recently.12
P. vulgaris inhabits the intestinal tract of humans and animals and can be found in soil, water and feces.8 Moreover, it was also observed in fecal samples of healthy individuals.13
P. mirabilis has developed resistance to various classes of antibiotics, such as β-lactams aminoglycosides and tetracyclines.14 However, P. mirabilis strains are generally more susceptible to antimicrobials than are P. vulgaris,
P. penneri, and P. hauseri.15
FISH technique was successfully used for clinical detection of Proteus spp. in artificial urine medium and urine samples from patients with UTIs. The probe was able to detect 11 strains of P.mirabilis, 6 strains of P. vulgaris, 2 strains of P.penneri and one strain of P. hauseri.16 The probe can also be used to detect P. mirabilis and P. vulgaris pure culture (as described in the figure legends). FISH can also be implicated to detect Proteus spp. in colon sections embedded in paraffin.17,18 Moreover, FISH can be implicated to identify Proteus spp. in the gut of the medicinal leech.19
Application
Sonda do fluorescencyjnej hybrydyzacji in situ (FISH), rozpoznaje komórki Proteus vulgaris i Proteus mirabilis.
Features and Benefits
- Wizualizacja, identyfikacja i izolacja komórek Proteus spp.
- Obserwacja natywnych populacji komórek Proteus spp. w różnych środowiskach mikrobiomu.
- Specyficzna, czuła i niezawodna identyfikacja Proteus spp. w mieszanej populacji bakterii.
- Specyficzna, czuła i niezawodna identyfikacja nawet w przypadku niskiej liczebności Proteus spp. w próbce.
- FISH może uzupełnić metody wykrywania oparte na PCR, unikając wykrywania bakterii zanieczyszczających.
- Dostarcza informacji na temat morfologii Proteus spp. i umożliwia badanie architektury biofilmu.
- Identyfikacja Proteus spp. w próbkach klinicznych, takich jak próbki moczu i utrwalone w formalinie próbki zatopione w parafinie (FFPE).
- Zdolność do wykrywania Proteus spp. w ich naturalnym środowisku jest niezbędnym narzędziem do badania interakcji gospodarz-mikrobiom.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| fluorescence λex 550 nm; λem 570 nm (Cy3) | fluorescence λex 504 nm; λem 521 nm (ATTO488) | fluorescence λex 550 nm; λem 570 nm | fluorescence λex 550 nm; λem 570 nm (Cy3) |
| technique(s) FISH: suitable | technique(s) FISH: suitable | technique(s) FISH: suitable | technique(s) FISH: suitable |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
Klasa składowania
12 - Non Combustible Liquids
wgk
nwg
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.