There is no specific data available for cell media with this kit. Phenol red is excited at 440 nm, so it should not interfere in this case. However, it might elevate background fluorescence, so it may be advisable to choose a cell media that does not contain phenol red.
Przejdź do
MAK272
High Sensitivity β-Hydroxybutyrate Fluorometric Assay Kit
sufficient for 100 fluorometric tests
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Informacje o tej pozycji
NACRES:
NA.25
UNSPSC Code:
12161503
application(s)
cosmetics
food and beverages
detection method
fluorometric
relevant disease(s)
endocrinological disorders, diabetes; gastrointestinal diseases
storage temp.
−20°C
General description
β -Hydroxybutyrate (β-HB), also known as 3-hydroxybutyrate, is a ketone body primarily synthesized in the liver from the oxidation of fatty acids. Ketone body formation, ketogenesis, is elevated when blood glucose levels drop. Moderately elevated blood ketone bodies occur normally during fasting and prolonged exercise. During alcoholic or diabetic ketoacidosis, blood levels of β -Hydroxybutyrate can rise to abnormally high levels. β -Hydroxybutyrate can be used as a marker for hepatic energy metabolism.
Application
Suitable for use with serum, plasma, urine, and other biological fluids, as well as food products such as milk.
Biochem/physiol Actions
The High Sensitivity β-Hydroxybutyrate Assay Kit is a simple, sensitive, high-throughput adaptable method for quantifying β-hydroxybutyrate when sample quantities are limited or sub-ketogenic levels are suspected. In this assay, β-hydroxybutyrate is enzymatically oxidized generating a fluorometric signal (lex = 535 nm/lem = 587 nm), proportional to the amount of β-hydroxybutyrate present. The assay is sensitive to 4 mM of β-hydroxybutyrate in a variety of biological samples.
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Klasa składowania
10 - Combustible liquids
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Is it possible to use MAK272 to detect β-Hydroxybutyrate in phenol red-containing media?
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