The copper assay will detect all free copper ions. It is not specific to Cu2+. That is simply what is used as the standard for the kit.
Przejdź do
MAK127
Copper Assay Kit
sufficient for 250 colorimetric tests
Synonim(y):
Copper Test Kit
Zaloguj się, aby wyświetlić ceny organizacyjne i kontraktowe.
Wybierz wielkość
Zmień widok
| Do Państwa/SKU | Dostępność | Cena netto |
|---|---|---|
1 kit | Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności | 2650,00 zł |
Informacje o tej pozycji
NACRES:
NA.84
UNSPSC Code:
12161503
2650,00 zł
Skontaktuj się z Obsługą Klienta, aby uzyskać informacje na temat dostępności
Pomoc techniczna
Potrzebujesz pomocy? Nasz zespół doświadczonych naukowców chętnie Ci pomoże.
Pozwól nam pomócusage
sufficient for 250 colorimetric tests
detection method
colorimetric
relevant disease(s)
orthopedic diseases; aging/geriatric diseases; hematological disorder; gastrointestinal diseases; neurological disorders; dermatological diseases
storage temp.
2-8°C
General description
Copper is an essential trace element. Copper-containing enzymes play important roles in iron and catecholamine metabolism, free radical scavenging, and in the synthesis of hemoglobin, elastin, and collagen. Copper is mainly present in ceruloplasmin in the liver. Low levels of copper have been associated with mental retardation, depigmentation, anemia, hypotonia, and scorbutic changes in bone. Levels of copper are key diagnostic indicator of diseases such as Wilson′s disease, microcytic hypochromic anemia, and bone disease due to reduced collagen synthesis.
Application
Copper Assay Kit has been used to determine the copper content colorimetrically in samples.
Suitable for the detection of of copper in biological, environmental, food, and beverage samples. Suitable for studying the effects of compounds on copper metabolism.
Biochem/physiol Actions
The method utilizes a chromogen that forms a colored complex specifically with copper ions. The intensity of the color, measured colorimetrically (359 nm), is directly proportional to copper concentration in the sample. The range of linear detection is 7 μg/dL (1.0 μM) to 300 μg/dL (47 μM).
Features and Benefits
Compatible with high-throughput handling systems. Can be adapted for use with cuvettes.
Ta strona może zawierać tekst przetłumaczony maszynowo.
1 of 1
Ta pozycja | |||
|---|---|---|---|
| description sufficient for 250 colorimetric tests | description sufficient for 250 colorimetric tests | description sufficient for 500 colorimetric tests | description sufficient for 100 colorimetric or fluorometric tests |
| usage sufficient for 250 colorimetric tests | usage sufficient for 250 colorimetric tests | usage sufficient for 500 colorimetric tests | usage sufficient for 100 colorimetric or fluorometric tests |
| detection method colorimetric | detection method colorimetric | detection method colorimetric | detection method fluorometric, colorimetric |
| storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. −20°C |
| relevant disease(s) orthopedic diseases; aging/geriatric diseases; hematological disorder; gastrointestinal diseases; neurological disorders; dermatological diseases | relevant disease(s) hematological disorder | relevant disease(s) gastrointestinal diseases; cardiovascular diseases | relevant disease(s) gastrointestinal diseases; cancer |
signalword
Danger
hcodes
Hazard Classifications
Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Met. Corr. 1 - Skin Corr. 1A - STOT SE 3
target_organs
Respiratory system
Klasa składowania
8B - Non-combustible corrosive hazardous materials
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
Wybierz jedną z najnowszych wersji:
Masz już ten produkt?
Dokumenty związane z niedawno zakupionymi produktami zostały zamieszczone w Bibliotece dokumentów.
-
Does this assay detect Cu+ or Cu2+ ions in the solution ?
1 answer-
Helpful?
-
-
如何检测细胞里的铜含量，需要预处理吗
1 answer-
Suspend about two million harvested cells in 400 ?L PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is helpful to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays.
Helpful?
-
-
How can I determine the shelf life / expiration / retest date of this product?
1 answer-
If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
-
-
该产品是否可以检测脑组织中的铜离子，脑组织是否需要特殊处理？
1 answer-
This assay kit has not been specifically tested on brain tissue. Researchers have published using this kit with brain tissue. Their sample preparation involved homogenizing brain tissue in distilled water prior to centrifugation and assaying the supernatant for copper content. Please see the citation below for more details.
Ma X, Sun Y, Li C, Wang M, Zang Q, Zhang X, Wang F, Niu Y, Hua J. Novel Insights Into DLAT's Role in Alzheimer's Disease-Related Copper Toxicity Through Microglial Exosome Dynamics. CNS Neurosci Ther. 2024 Oct;30(10):e70064. doi: 10.1111/cns.70064. PMID: 39428563; PMCID: PMC11491298.
Helpful?
-
-
How is shipping temperature determined? And how is it related to the product storage temperature?
1 answer-
Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
Helpful?
-
-
How to homogenize the mouse liver, for example, in what buffer, to measure copper content using the MAK127 Copper Assay Kit?
1 answer-
This is the closest protocol for liver homogenate for use of the kit with cell lysates (presumably cultured cells):
- Lyse cells in 0.5 N NaOH for 30 minutes on a shaking plate followed by a 30-minute incubation at 100 degrees C.
- Clarify the lysate by centrifugation (2 min at 14,000 rpm) and use the clear supernatant for the assay.
- Avoid the use of divalent chelators like EDTA during the preparation.
It indicates that Reagent A contains Trichloroacetic acid (concentration not provided), so it should neutralize the NaOH in the lysate. It is recommended to review the kit's Technical Bulletin regarding the possible need to centrifuge samples that contain proteins.Helpful?
-
-
Does this product solely detect free copper in the solution, or does it also measure bound copper, such as Ceruloplasmin-bound copper?
1 answer-
This kit is not specifically validated for ceruloplasmin-bound copper, But, it is believed that the assay can measure ceruloplasmin-bound copper because it uses a very strong copper chelate. Any other means to dissociate the Cu (such as heating to denature protein) would be helpful, although they may not be necessary.
Helpful?
-