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MAK057

Citrate Assay Kit

sufficient for 100 colorimetric or fluorometric tests

Synonim(y):

Citrate Test Kit

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Informacje o tej pozycji

NACRES:
NA.84
UNSPSC Code:
12161503

usage

sufficient for 100 colorimetric or fluorometric tests

application(s)

cosmetics
food and beverages

detection method

colorimetric, fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Citrate is a key Tricarboxylic Acid (TCA) cycle intermediate formed by the addition of oxaloacetate to the acetyl group of acetyl-CoA. Citrate is transported out of the mitochondria via the citrate-malate shuttle and converted back to acetyl-CoA for fatty acid synthesis. Citrate is an allosteric modulator of both fatty acid synthesis via its actions on acetyl-CoA carboxylase and of glycolysis via its actions on phospho- fructokinase. Citrate metabolism and disposition can vary widely due to sex, age, and a variety of other factors including disease states. Cellular citrate levels are decreased in prostrate cancer cells and citrate levels may be a marker of prostrate cancer growth rate. The Citrate Assay Kit provides a simple, sensitive, and rapid means of quantifying citrate in a variety of samples.

Application

Citrate Assay kit has been used to determine the concentration of citrate in samples.
Suitable for the measurement of citrate in a variety of samples including tissue and cells

Biochem/physiol Actions

Citrate concentration is determined by a coupled enzyme assay, which results in a colorimetric (570 nm)/ fluorometric (λex = 535/λem = 587 nm) product, proportional to the citrate present. Typical sensitivites of the Citrate Assay Kit are between 0.1 to 10 nmoles (2 μM-10 mM) of citrate in a variety of samples.
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Klasa składowania

10 - Combustible liquids

wgk

WGK 3

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

signalword

Danger

Hazard Classifications

Aquatic Chronic 3 - ED ENV 1

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Certyfikaty analizy (CoA)

Lot/Batch Number

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Questions

1–3 of 3 Questions  

  1. How can I determine the shelf life / expiration / retest date of this product?

    1 answer

    1. If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
      For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
      For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
      For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdf

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  2. How is shipping temperature determined? And how is it related to the product storage temperature?

    1 answer

    1. Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf

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  3. How are the detection range and sensitivity determined for the MAK057 assay?

    1 answer

    1. The calculation of the higher limit of detection is determined by dividing the highest concentration of the standard by the minimum sample volume. Similarly, the lower limit of detection is calculated by dividing the lowest concentration standard by the maximum sample volume. In the event that a sample exceeds the range of the standard curve, it is advisable to conduct a dilution and then adjust for the dilution factor.
      For instance, for the MAK057 test:

      Colorimetric:
      • Lower range: 2 nmol/50 = 0.04 mM or 40 uM
      • Higher range: 10 nmol/1 uL = 10 mM or 10,000 uM
      Fluorometric:
      • Lower range: 0.2 nmol/50 = 0.004 mM or 4 uM
      • Higher range: 1 nmole/1 uL = 1 mM or 1000 uM

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