M9015

Malachite Green oxalate salt

Dye content, ≥90%, certified by the Biological Stain Commission, powder or crystals

• Synonim(y): N,N,N′,N′-Tetramethyl-4,4′-diaminotriphenylcarbenium oxalate, Basic Green 4
• Wzór empiryczny (zapis Hilla): C₂₃H₂₅N₂ · C₂HO₄ · 0.5C₂H₂O₄
• Numer CAS: 2437-29-8
• Masa cząsteczkowa: 463.50
• Numer w indeksie barw: 42000
• Beilstein: 3644550
• Numer WE: 219-441-7
• Kod UNSPSC: 12171500
• Identyfikator substancji w PubChem: 24897308
• NACRES: NA.47

Właściwości

• Nazwa produktu: Malachite Green oxalate salt, certified by the BSC
• agency: certified by the BSC
• Formularz: powder or crystals
• skład: Dye content, ≥90%
• metody: microbe id | staining: suitable
• kolor: faint green to very dark green
• mp: 144-150 °C
• rozpuszczalność: H₂O: 1 mg/mL, blue to very deep blue
• Zastosowanie: diagnostic assay manufacturing; hematology; histology
• temp. przechowywania: room temp
• ciąg SMILES: C[N+](C)=C1C=CC(C=C1)=C(C2=CC=C(N(C)C)C=C2)C3=CC=CC=C3.[O-]C(C(O)=O)=O
• InChI: 1S/C23H25N2.C2H2O4/c1-24(2)21-14-10-19(11-15-21)23(18-8-6-5-7-9-18)20-12-16-22(17-13-20)25(3)4;3-1(4)2(5)6/h5-17H,1-4H3;(H,3,4)(H,5,6)/q+1;/p-1
• Klucz InChI: REZFCYOVFCJTDG-UHFFFAOYSA-M

Opis

Opis ogólny

Malachite Green is a basic dye belonging to the triphenylmethane group. It is required in biology for the staining of microscopic samples, such as plant tissue, yeast and bacteria. The malachite green method is a sensitive method for the detection of released phosphate.[1][2]

Zastosowanie

Malachite Green oxalate salt has been used for phosphate quantification.[3][4]

Malachite Green oxalate salt has been used:

• in the preparation of Alexander staining solution for cell viability test[5]
• in malachite green-ammonium molybdate assay for determining the adenosine triphosphatase (ATPase) activity of binding immunoglobulin protein (BiP)[6]
• as a hydrophobic ion, to study the label-free molecular transport in the living cell membranes of Escherichia coli in real time by second-harmonic generation (SHG) method[7]

Przydatność

Certified for use as a counterstain for paraffin embedded botanical material; as a spore stain for bacteria by the method of Schaeffer and Fulton.

Inne uwagi

The molecular mass of malachite green oxalate is expressed as either:
927.00 g/mol - the mass of 2 mol malachite green + 2 mol monohydrogen oxalate + 1 mol dihydrogen oxalate, or
463.50 g/mol - the mass of 1 mol malachite green + 1 mol monohydrogen oxalate + 0.5 mol dihydrogen oxalate

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Informacja o środkach bezpieczeństwa

• Piktogramy: GHS06,GHS08,GHS05,GHS09
• Hasło ostrzegawcze: Danger
• Zwroty wskazujące rodzaj zagrożenia: H301,H318,H361fd,H410
• Zwroty wskazujące środki ostrożności: P202 - P264 - P273 - P280 - P301 + P310 - P305 + P351 + P338
• Klasyfikacja zagrożeń: Acute Tox. 3 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Eye Dam. 1 - Repr. 2
• Kod klasy składowania: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Klasa zagrożenia wodnego (WGK): WGK 3
• Temperatura zapłonu (°F): Not applicable
• Temperatura zapłonu (°C): Not applicable