M8284

Maltose Phosphorylase from Enterococcus sp.

Name: Maltose Phosphorylase from <I>Enterococcus</I> sp.
Brand: SIGMA
Price: 800 PLN

recombinant, expressed in E. coli, lyophilized powder

Synonim(y):

Maltose:orthophosphate 1-β-D-Glucosyltransferase

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Informacje o tej pozycji

Numer CAS:

9030-19-7

UNSPSC Code:

12352204

PubChem Substance ID:

24897239

NACRES:

NA.54

Numer WE:

2.4.1.8 (BRENDA, IUBMB)

MDL number:

MFCD03457426

Specific activity:

≥9 units/mg solid

Recombinant:

expressed in E. coli

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SMILES string

OC(=O)CCCCCCCCCCCCC

InChI key

TUNFSRHWOTWDNC-UHFFFAOYSA-N

InChI

1S/C14H28O2/c1-2-3-4-5-6-7-8-9-10-11-12-13-14(15)16/h2-13H2,1H3,(H,15,16)

recombinant

expressed in E. coli

form

lyophilized powder

specific activity

≥9 units/mg solid

mol wt

90 kDa by SDS-PAGE

storage temp.

−20°C

Quality Level

200

Powiązane kategorie

Glycobiology Profiling Tools

Protein Biology

Proteins & Enzymes

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Ta pozycja	SAE0051	S0937	N8264
Sigma-Aldrich M8284 Maltose Phosphorylase from Enterococcus sp.	Sigma-Aldrich SAE0051 Glycerol 3-phosphate Oxidase from Enterococcus sp.	Sigma-Aldrich S0937 Sucrose Phosphorylase	Sigma-Aldrich N8264 Nucleoside Phosphorylase from microorganisms
specific activity ≥9 units/mg solid	specific activity ≥35 unit/mg solid	specific activity ≥45 units/mg solid	specific activity ≥10 units/mg protein
recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant expressed in E. coli	recombinant -
form lyophilized powder	form lyophilized powder	form lyophilized powder	form lyophilized powder
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
mol wt 90 kDa by SDS-PAGE	mol wt -	mol wt 56 kDa by SDS-PAGE	mol wt ~120 kDa
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

Maltose phosphorylase (MP) is a dimeric enzyme. This enzyme is classified under family 65 of the glycoside hydrolases. It is a member of the disaccharide phosphorylase family.[1]

Application

Maltose Phosphorylase from Enterococcus sp. has been used as a component in coupled assay and ATPase activity assay to study its effects on ATPase activity, on the 90 kDa heat shock proteins (Hsp90) ATPase reaction and on background absorbance on the production of resorufin.[2]

Maltose phosphorylase from Enterococcus has been used in a study to describe a new pathway for maltose utilization in lactic acid bacteria.[3] It has also been used in a study to describe the transfer of glucosyl moiety of maltose to acceptors with alcoholic OH groups.[4]

Biochem/physiol Actions

Enzymatically converts maltose to D-Glucose.

Maltose phosphorylase (MP) is a dimeric enzyme that catalyzes maltose and inorganic phosphate into β-D-glucose-1-phosphate and glucose. [1]

Maltose phosphorylase not only transfers glucosyl moieties from maltose to other sugars but has been shown to also use phenolic compounds such as salicyl alcohol as acceptors.[4]

Other Notes

Contains lactose.

One unit will produce 1.0 μmole of D-Glucose from maltose per minute at pH 7.0 at 30°C.

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Klasa składowania

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Odwiedź Bibliotekę dokumentów

Purification and characterization of maltose phosphorylase from Bacillus sp. RK-1.

Y Inoue et al.

Bioscience, biotechnology, and biochemistry, 65(12), 2644-2649 (2002-02-06)

Bacillus sp. RK-1 was isolated as a bacterium that produced maltose phosphorylase (MPase) in the culture supernatant. Screening was done from among about 400 isolates that could grow at 55 degrees C in a medium containing maltose as the sole

In-gel precipitation of enzymatically released phosphate.

Ana D Simonović et al.

Analytical biochemistry, 334(2), 312-317 (2004-10-21)

The phosphate precipitation reaction using ammonium molybdate and triethylamine under low pH has been applied to gel-based assays for detecting phosphate-releasing enzymes. The sensitivity of the assay is 10 pmol Pi/mm2 of 1.5-mm-thick gel. The assay is applicable to enzymes

Development of a conductometric phosphate biosensor based on tri-layer maltose phosphorylase composite films.

Zhiqiang Zhang et al.

Analytica chimica acta, 615(1), 73-79 (2008-04-29)

A conductometric biosensor for phosphate detection was developed using maltose phosphorylase (MP) from recombinant Escherichia coli immobilized on a planar interdigitated electrode by cross-linking with saturated glutaraldehyde (GA) vapour in the presence of bovine serum albumin (BSA). The process parameters

The maltodextrin transport system and metabolism in Lactobacillus acidophilus NCFM and production of novel alpha-glucosides through reverse phosphorolysis by maltose phosphorylase.

Hiroyuki Nakai et al.

The FEBS journal, 276(24), 7353-7365 (2009-11-19)

A gene cluster involved in maltodextrin transport and metabolism was identified in the genome of Lactobacillus acidophilus NCFM, which encoded a maltodextrin-binding protein, three maltodextrin ATP-binding cassette transporters and five glycosidases, all under the control of a transcriptional regulator of

Cloning of the maltose phosphorylase gene from Bacillus sp. strain RK-1 and efficient production of the cloned gene and the trehalose phosphorylase gene from Bacillus stearothermophilus SK-1 in Bacillus subtilis.

Yasushi Inoue et al.

Bioscience, biotechnology, and biochemistry, 66(12), 2594-2599 (2003-02-25)

The maltose phosphorylase (MPase) gene of Bacillus sp. strain RK-1 was cloned by PCR with oligonucleotide primers designed on the basis of a partial N-terminal amino acid sequence of the purified enzyme. The MPase gene consisted of 2,655 bp encoding

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Maltose Phosphorylase from Enterococcus sp.