M2154

Medium 199

With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture

• Synonim(y): M199 Medium, TCM 199
• UNSPSC Code: 12352207
• NACRES: NA.75

Właściwości

• Nazwa produktu: Medium 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
• Quality Segment: 400
• sterility: sterile-filtered
• form: liquid
• technique(s): cell culture | mammalian: suitable
• impurities: endotoxin, tested
• pH: 7.0-7.6 (25 °C, 209 g/L)
• components: sodium pyruvate: no; L-glutamine: no; NaHCO₃: yes; HEPES: no; phenol red: yes; Earle’s salts (5% CO₂): yes
• shipped in: ambient
• storage temp.: 2-8°C

Opis

General description

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

Application

Medium 199 has been used:

• to culture rumen epithelial cells from yearling Speckle Park beef heifers[1]
• as a component of the maturation medium for the incubation of Cumulus oocyte complexes (COCs) from bovine ovaries[2]
• as a component of the Dulbecco′s modified Eagle medium (DMEM) mixture to maintain rat neonatal cardiac myocytes (NCM)[3]

Preparation Note

Supplement with 0.1 g/L L-glutamine.

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Informacja o środkach bezpieczeństwa

• Klasa składowania: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable